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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections, in vitro blocking of ligand binding1, and immunoprecipitation1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 105608).
Application References:
1. Xu H, et al. 1996. J. Immunol. 156:4909. 2. Gerwin N, et al. 1999. Immunity 10:9.
C57BL/6 mouse splenocytes stained with biotinylated 3C4, followed by Sav-PE
CD102 is a 55 kD immunoglobulin superfamily member, also known as ICAM-2. CD102 is a heavily glycosylated cell surface ligand for LFA-1 (CD11a/CD18). CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and T cell activation. The 3C4(MIC2/4) antibody can block interactions between ICAM-2 and LFA-1.
Other Names:
ICAM-2
Structure:
Ig superfamily, 55 kD
Distribution:
Vascular endothelial cells, some resting lymphocytes, monocytes, platelets
Function:
Lymphocyte recirculation, costimulation
Ligand Receptor:
LFA-1, Mac-1
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017. 3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD102
C57BL/6 mouse splenocytes stained with biotinylated 3C4, followed by Sav-PE
FITC anti-mouse CD102
C57BL/6 mouse splenocytes stained with 3C4 FITC
Purified anti-mouse CD102
C57BL/6 mouse splenocytes stained with purified 3C4,followed by anti-rat IgG FITC
Alexa Fluor® 488 anti-mouse CD102
C57BL/6 mouse splenocytes stained with 3C4 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD102
C57BL/6 mouse splenocytes stained with 3C4 Alexa Fluor® 647
