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This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation:
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1-4 μg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Description:
Ku80 is an 80 kD nuclear ATP-dependent DNA helicase. Ku80 is involved in dsDNA break repair, VDJ recombination, and chromosome translocation. This protein binds fork-like ends of dsDNA in cell-cycle dependent manner. Ku80 can be modified by phosphorylation and has been shown to interact with Ku70 and DNA-PKcs. The F3 monoclonal antibody recognizes human Ku80 and has been shown to be useful for Western blotting and immunofluorescence staining.
Other Names:
ATP-dependent DNA helicase II, Lupus Ku autoantigen protein
Binds fork-like ends of dsDNA in cell-cycle dependent manner
Modification:
Phosphorylation
Interaction:
Ku70, DNA-PKcs
Antigen References:
1. Yaneva M, et al. 1989. J. Biol. Chem. 264:13407. 2. Stuiver M, et al. 1990. J Exp Med. 172:1049. 3. Tuteja N, et al. 1994. EMBO J. 13:4991. 4. Song J, et al. 2003. J. Biol. Chem. 278:36676.
Biotin anti-Ku80, F3, Biotin, ATP-dependent DNA helicase II, Lupus Ku autoantigen protein
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