Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: Immunofluorescence cell staining1, immunoprecipitation1, Western blotting1-2, ELISA1 and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.
Application References:
1. Andrews, P.W., et al., 1984. Hybridoma. 3: 347. 2. Schopperle, W.M. and Dewolf, W.C., 2007. Stem Cells. 25: 723.
Human teratocarcinoma cell line, NCCIT, stained with biotinylated TRA-1-81, followed by Sav-PE
Description:
The TRA-1-81 antibody reacts with a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-81 (Tumor Rejection Antigen 1-81) on human ESC is downregulated upon differentiation. Further studies have shown that TRA-1-81 is neuroaminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of mucin-like glycoprotein with CD34 family. Podocalyxin is a type I tranmembrane sialoprotein with anti-adhesion property.
Other Names:
Podocalyxin
Structure:
Mucin-like protein, CD34 family member, 200-240 kD
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