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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: T cell activation.
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. (FC) 2. Joseph A, et al. 2008. J. Virol. 82:3078. (FC) PubMed
Human peripheral blood lymphocytes stained with biotinylated IP26, followed by Sav-PE
The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human TCR α/β
Human peripheral blood lymphocytes stained with biotinylated IP26, followed by Sav-PE
FITC anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 FITC
PE anti-human α/β T Cell Receptor
Human peripheral blood lymphocytes stained with IP26 PE
PE/Cy5 anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 PE/Cy5
Purified anti-human TCR α/β
Human peripheral blood lymphocytes stained
with purified IP26, then detected
with anti-mouse IgG FITC
PE/Cy7 anti-human TCR α/β
Human peripheral blood lymphocytes stained with CD19 (HIB19) FITC and IP26 PE/Cy7 (top) or mouse IgG1, κ PE/Cy7 isotype control (bottom)
Alexa Fluor® 488 anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 Alexa Fluor® 488
Alexa Fluor® 647 anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 Alexa Fluor® 647
Pacific Blue™ anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 Pacific Blue™
APC anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 APC
