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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemistry of acetone-fixed frozen sections. ITEM-4 antibody has no effect on TWEAK-induced MAPK activation in RAW cells1.
Application References:
1. Polek TC, et al. 2003. J. Biol. Chem. 278:32317. 2. Nakayama M, et al. 2003. J. Immunol. 170:341.
Human Fn14 transfected cells stained with biotinylated ITEM-4, followed by Sav-PE
CD266 is a 14 kD transmembrane protein known as TWEAK receptor (TWEAK-R) or fibroblast growth factor inducible 14 (Fn14). It is member 12A of the tumor necrosis factor receptor superfamily (TNFRSF12A) expressed at low levels in a variety of tissues, such as heart, placenta, lung, muscle, and pancreas. TWEAK-R binds TWEAK (CD255) and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The engagement of Fn14 can induce cell death and may play a role in inflammation, hepatocyte growth, and liver neoplasia.
Transmembrane TNF superfamily receptor, contains no death domain, 14 kD
Distribution:
Widely expressed in heart, placenta, lung, muscle, kidney, pancreas, and HUVEC
Function:
Binds to TWEAK (CD255), thought to play a role in cell death and inflammation
Ligand Receptor:
TWEAK (CD255); interacts with cytoplasmic proteins TRAF1, 2, and 3
Antigen References:
1. Feng SL, et al. 2000. Am. J. Path. 156:1253. 2. Meighan-Mantha RL, et al. 1999. J. Biol. Chem. 274:33166. 3. Nakayama M, et al. 2003. J. Immunol. 170:341. 4. Lynch CN, et al. 1999. J. Biol. Chem. 274:8455.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human/mouse CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with purified ITEM-4, followed by anti-mouse IgG FITC
Biotin anti-human/mouse CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with biotinylated ITEM-4, followed by Sav-PE
PE anti-human/mouse CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with ITEM-4 PE
