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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-8 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA Detection: The biotinylated E8N1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H8A5 (Cat. No. 511502) antibody as the capture antibody. Flow Cytometry: The fluorochrome-labeled E8N1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-8-producing cells within mixed cell populations. View intracellular cytokine staining protocol Note: For testing human IL-8 in serum or plasma, BioLegend's ELISA Max™ Sets are specially developed and recommended.
IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The E8N1 antibody recognizes the human IL-8 protein and has been shown to be useful for intracellular immunofluorescence flow cytometric analysis and as ELISA detection antibody.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human IL-8
FITC anti-human IL-8
LPS stimulated (6hrs) and monensin treated human peripheral blood monocytes surface stained with HCD14 APC and intracellularly stained with E8N1 FITC
PE anti-human IL-8
LPS-stimulated (6 hours, in the presence of monensin) human peripheral blood monocytes intracellularly stained with E8N1 PE
Alexa Fluor® 488 anti-human IL-8
LPS-stimulated (6 hours, in the presence of monensin) human peripheral blood monocytes intracellularly stained with E8N1 Alexa Fluor® 488
