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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5-2.0 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-6 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA or ELISPOT Detection1-4: The biotinylated MQ2-39C3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified MQ2-13A5 antibody (Cat. No. 5001102/501110) as the capture antibody. Additional reported applications (for the relevant formats) include: neutralization1, Western blotting. The LEAF™ purified MQ2-13A5 antibody (Endotoxin Note: For testing human IL-6 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430501 to 430506) are specially developed and recommended.
Application References:
1. Abrams, J., et al. 1992. Immunol. Rev. 127:5. 2. Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20. 3. Gaines Das, R., et al. 1993. J. Immunol. Meth. 160:147. 4. Lesmeister, M. J., et al. 2005. Reproductive Biology and Endocrinology 3:74.
IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. The MQ2-39C3 antibody can neutralize the bioactivity of natural or recombinant IL-6.
Other Names:
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Structure:
Neuropoietic cytokine family; 26 kD (Mammalian)
Regulation:
Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Cellular Sources:
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cellular Targets:
T cells, B cells, hepatocytes, cholinergic neurons
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Hirano, T. 1998. Intl. Rev. Immunol. 16:249. 3. Patterson, P. 1992. Curr. Opin. Neurobiol. 2:94. 4. Van Oers, M., et al. 1993. Ann. Hematology 66:219.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
