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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection, dilute 60 µl of the biotinylated anti-human IL-2 in 12 ml of assay diluent for each plate. To obtain a linear standard curve, serial dilutions of IL-2 recombinant protein ranging from 15.6 to 1000 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA Detection: The biotinylated Poly5176 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified MQ1-17H12 (Cat. No. 500302) antibody as the capture antibody.
Note: For testing human IL-2 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431801 to 431806) are specially developed and recommended.
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
Other Names:
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Structure:
Cytokine; 15.4 kD (Mammalian)
Regulation:
Upregulated by NFAT; downregulated by TCF-8, CIF (colostrums inhibitory factor)
Cellular Sources:
T cells
Cellular Targets:
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors:
High affinity heterotrimer of IL-2Ra/b/g, intermediate affinity homodimer IL-2Ra (CD25; p55; Tac) and heterodimer IL-2Rb (CD122)/g; g-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Bioactivity/Activities:
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Taniguchi T, et al. 1993. Cell 73:5. 3. Nistico G. 1993. Prog. Neurobiol. 40:463. 4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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