The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Application:
ELISA Detection, ELISPOT Detection
Recommended Usage:
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-1β protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 2-8 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
ELISA or ELISPOT Detection: The biotinylated JK1B-2 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JK1B-1 antibody (Cat. No. 508202/508204) as the capture antibody.
Description:
IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The JK1B-2 antibody reacts with human interleukin-1β (IL-1β) and baboon IL-1β. The JK1B-2 antibody does not recognize mouse IL-1β or human IL-1α or other cytokines. The JK1B-2 antibody can neutralize the bioactivity of natural or recombinant IL-1β.
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Cellular Sources:
Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Cellular Targets:
B cells, T cells, monocytes
Receptors:
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Bioactivity/Activities:
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
Antigen References:
1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Bomford, R., et al., Eds. 1989. Interleukin-1, inflammation and disease. Elsevier, New York. 3. Brazel, D., et al., 1991. Biotechnol. Ther. 2:241. 4. Dinarello, C. 1996. Blood 87:2095.
