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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by ELISA assay. As a detection antibody in ELISA assays, dilute 60 µl of this biotinylated antibody into 12 ml of detection buffer for one 96 well microtiter plate assay. To obtain a linear standard curve, serial dilutions of human IL-13 recombinant protein ranging from 2000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA Detection or ELISPOT Detection: The biotinylated Poly5020 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES10-5A2 (Cat. No. 501902/501910) antibody as the capture antibody.
Application References:
1. Bailer, R.T., et al.., 1999. J. Immunol.. 162 :7534. 2. Arva, E., et al.., 1999. Scand. J. Immunol.. 49:411.
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The poly5020 polyclonal antibody reacts with human interleukin-13 (IL-13).
Other Names:
Interleukin-13, NC30
Structure:
Cytokine; 12.5 kD
Function:
Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
Regulation:
IL-13Rα2 possible antagonist
Cellular Sources:
Activated T helper cells, CD8+ T cells, mast cells, NK cells
Cellular Targets:
B cells, monocytes, macrophages, basophils
Receptors:
Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
Bioactivity/Activities:
Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
Antigen References:
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497. 3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797. 4. Ochensberger, B., et al. 1996. Blood 88:3028.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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