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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen sections and formalin-fixed paraffin-embedded sections1, and immunoprecipitation1.
The LN3 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.
Other Names:
Major Histocompatibility Class II, MHC class II
Structure:
Ig superfamily, MHC class II, heterodimeric transmembrane protein
Distribution:
B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
Function:
Peptide presentation
Ligand Receptor:
CD3/TCR, CD4
Antigen References:
1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177. 2. Terstappen L, et al. 1990. J. Leuk. Biol. 48:138. 3. Edwards J, et al. 1985. J. Immunol. 137:490. 4. van Es A, et al. 1984. Transplantation 37:65. 5. O'Doherty U, et al. 1994. Immunology 82:487. 6. Thomas R, et al. 1994. J. Immunol. 153:4016. 7. Grouard G, et al. 1996. Nature 384:364.
Biotin anti-human HLA-DR, LN3 , Biotin, Major Histocompatibility Class II, MHC class II, Human, Cross-Reactivity*: Rhesus, Flow Cytometry, Immunohistochemistry, Immunology, Antibodies
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human HLA-DR
Human peripheral blood lymphocytes stained with purified LN3, followed by anti-mouse IgG FITC
Biotin anti-human HLA-DR
Human peripheral blood lymphocytes stained with biotinylated LN-3, followed by Sav-PE
FITC anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 FITC
PE anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 PE
Alexa Fluor® 488 anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 Alexa Fluor® 488
Alexa Fluor® 647 anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 Alexa Fluor® 647
Alexa Fluor® 700 anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN3 Alexa Fluor® 700
Pacific Blue™ anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN3 Pacific Blue™
