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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human FGF-basic protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate.It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA or ELISPOT Detection: The biotinylated JKFb-2 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JKFb-1 antibody (Cat. No. 508602/508604) as the capture antibody.
Fibroblast growth factor-basic (FGF-b, FGF-2) is a heparin-binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-basic also exerts a potent angiogenic activity in vivo. FGF-basic has been isolated from neural, pituitary, adrenal cortex, and placental tissues. The JKFb-2 antibody reacts with human fibroblast growth factor - basic (FGF-basic).
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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