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Biotin anti-human CD4 Antibody
Biotin anti-human CD4 Antibody
300504 100 µg $160.00     

Product Details

 
Clone: RPA-T4 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Biotin Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
IV T114
Reactivity: Human, Cross-Reactivity: Chimpanzee
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Application
Notes:

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry3,4,5 of acetone-fixed frozen sections, and blocking of T cell activation1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300516).

Application
References:

  Publication Library
Biotin anti-human CD4 Antibody RPA-T4 Image 1
Human peripheral blood lymphocytes stained with biotinylated RPA-T4, followed by Sav-PE


Compare all formats



Description:

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Other Names: T4
Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand Receptor: MHC class II molecules, HIV gp120, IL-16
Antigen
References:

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

GeneID: 920
Latest Publications: View the latest CD4 articles on HighwirePress.com
UniProt: View information about CD4 on UniProt.org
Keywords: Biotin anti-human CD4, RPA-T4, Biotin, T4, Human, Cross-Reactivity: Chimpanzee, Flow Cytometry, Immunology, Antibodies
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Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-human CD4RPA-T4FC
Alexa Fluor® 647 anti-human CD4RPA-T4FC
Alexa Fluor® 700 anti-human CD4RPA-T4FC
APC anti-human CD4RPA-T4FC
APC/Cy7 anti-human CD4RPA-T4FC
Brilliant Violet 421™ anti-human CD4RPA-T4FC
Brilliant Violet 570™ anti-human CD4RPA-T4FC
FITC anti-human CD4RPA-T4FC
LEAF™ Purified anti-human CD4RPA-T4Block, FC, IHC, CyTOF®
Pacific Blue™ anti-human CD4RPA-T4FC
PE anti-human CD4RPA-T4FC
PE/Cy5 anti-human CD4RPA-T4FC
PE/Cy7 anti-human CD4RPA-T4FC
PerCP anti-human CD4RPA-T4FC
PerCP/Cy5.5 anti-human CD4RPA-T4FC
Purified anti-human CD4RPA-T4FC, IHC, CyTOF®

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PE

  • Biotin anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated RPA-T4, followed by Sav-PE

  • FITC anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 FITC

  • LEAF™ Purified anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified RPA-T4, followed by anti-mouse IgGs FITC

  • PE anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PE

  • PE/Cy5 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PE/Cy5

  • PE/Cy7 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PE/Cy7

  • Purified anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified RPA-T4, followed by anti-mouse IgGs FITC

  • APC/Cy7 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 APC/Cy7

  • Alexa Fluor® 488 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 Alexa Flour® 647

  • Pacific Blue™ anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 Pacific Blue™

  • Brilliant Violet 421™ anti-human CD4
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD4 (clone RPA-T4) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).





  • Alexa Fluor® 700 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 Alexa Fluor® 700

  • PerCP anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PerCP

  • PerCP/Cy5.5 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PerCP/Cy5.5

  • Brilliant Violet 570™ anti-human CD4
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD4 (clone RPA-T4) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).





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