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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 320810).
Application References:
1. Wu J, et al. 1999. Science 285:730. 2. Wu J, et al. 2000. J. Exp. Med. 192:1059. 3. Groh V, et al. 2001. Nature Immunol. 2:255. 4. Wu J, et al. 2002. J. Immunol. 169:1236. 5. Roberts A, et al. 2001. J. Immunol. 167:5527. 6. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452. 7. Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed
Human peripheral blood lymphocytes were stained with biotinylated CD314 (clone 1D11) (filled blue histogram), or biotinylated mouse IgG1, κ (open purple histogram), followed by Sav-PE.
NKG2D is a homodimeric C-type lectin-like protein also known as CD314. It is expressed on NK cells, CD8+ T cells, γ/δ T cells, and in vitro induced LAK cells. Several molecules have been identified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA), MICB, UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, but attains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to be a primary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCR-mediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligands plays a role in the immune surveillance against pathogen and tumor cells, and in the pathogenesis of autoimmune diseases. The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation.
Structure:
C-type lectin
Distribution:
NK cells, γ/δ T cells, CD8+ T cells
Function:
cytolytic killing of target cells expressing NGK2D ligands, costimulation of NK cells and T cells
Ligand Receptor:
MICA, MICB, UL16-binding proteins (ULBPs)
Antigen References:
1. Vance RE, et al. 1999. J. Exp. Med. 190:1801. 2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781. 3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755. 4. Jamieson AM, et al. 2002. Immunity 17:19. 5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. 6. Ho EL, et al. 2002. J. Immunol. 169:3667. 7. Maasho K, et al. 2005. J. Immunol. 174:4480. 8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD314 (NKG2D)
Human peripheral blood lymphocytes were stained with purified CD314 (clone 1D11) (filled histogram), or purified mouse IgG1, κ (open histogram), followed by anti-mouse IgG FITC.
Biotin anti-human CD314 (NKG2D)
Human peripheral blood lymphocytes were stained with biotinylated CD314 (clone 1D11) (filled blue histogram), or biotinylated mouse IgG1, κ (open purple histogram), followed by Sav-PE.
PE anti-human CD314 (NKG2D)
Human peripheral blood lymphocytes were stained with CD314 (clone 1D11) PE and CD8 PE/Cy5.
APC anti-human CD314 (NKG2D)
Human peripheral blood lymphocytes were stained with CD314 (clone 1D11) APC and CD56 PE.
LEAF™ Purified anti-human CD314 (NKG2D)
Human peripheral blood lymphocytes were stained with LEAF™ purified CD314 (clone 1D11) (filled histogram), or LEAF™ purified mouse IgG1, κ (open histogram), followed by anti-mouse IgG FITC.
PE/Cy7 anti-human CD314 (NKG2D)
Human peripheral blood lymphocytes were stained with CD56 APC and CD314 (clone 1D11) PE/Cy7 (bottom), or mouse IgG1, κ isotype control (top).
