Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections4, and in vitro T cell costimulation5-8 . The CD28.2 antibody co-stimulates T cell proliferation and cytokine production in the presence of suboptimal amounts of anti-CD3 antibody. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 302914). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 302934) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Nunes J, et al. 1993. Biochem. J. 293:835. 3. Calea-Lauri J, et al. 1999. J. Immunol. 163:62. 4. Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC) 5. Marti F, et al. 2001. J. Immunol. 166:197. (Costim) 6. Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim) 7. Rivollier A, et al. 2004. Blood 104:4029. (Costim) 8. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim) 9. Sheng W, et al. 2007.Elsevier 580:6819. PubMed 10. Mitsuhashi M. 2007. Clin Chem.53:148. PubMed 11. Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed 12. Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed 13. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 14. Rout N, et al. 2010. PLoS One 5:e9787. (FC) 15. Leonard JA, et al. 2011. J. Virol. 85:6867. PubMed
Human peripheral blood lymphocytes stained with biotinylated CD28.2, followed by Sav-PE
CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
Other Names:
T44, Tp44
Structure:
Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution:
Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
Function:
T cell costimulation
Ligand Receptor:
CD80, CD86
Antigen References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. June CH, et al. 1994. Immunol. Today 15:321. 3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD28
Human peripheral blood lymphocytes stained with APC CD28.2
Biotin anti-human CD28
Human peripheral blood lymphocytes stained with biotinylated CD28.2, followed by Sav-PE
FITC anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 FITC
LEAF™ Purified anti-human CD28
Human peripheral blood lymphocytes stained with LEAF™ purified CD28.2, followed by anti-mouse IgGs FITC
PE anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 PE
PE/Cy5 anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 PE/Cy5
Purified anti-human CD28
Human peripheral blood lymphocytes stained
with CD28.2 PE
Alexa Fluor® 488 anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 Alexa Fluor® 488
Alexa Fluor® 700 anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 Alexa Fluor® 700
PerCP/Cy5.5 anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 PerCP/Cy5.5
Pacific Blue™ anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 Pacific Blue™
PE/Cy7 anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 PE/Cy7
Ultra-LEAF™ Purified anti-human CD28
Human peripheral blood lymphocytes stained with LEAF™ purified CD28.2, followed by anti-mouse IgGs FITC
