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The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported application (with relevant formats) include: inhibition of T cell proliferation and cytokine production1.
Application References:
1. Otsuki N, et al. 2006. Biochem. Bioph. Res. Co. 344:1121. 2. Okano M, et al. 2008. Clin. Exp. Allergy 38:1891.
Human peripheral blood lymphocytes stained with CD3 (UCHT1) APC and biotinylated MIH26 (top) or biotinylated mouse IgG2a, κ isotype control (bottom), followed by Sav-PE
B and T lymphocyte attenuator (BTLA) is an Ig superfamily coinhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand Herpes Virus Entry Mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells. BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significant up-regulated expression of BTLA compared with effector and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It has been reported that BTLA may regulate T cell function through binding to B7-H4.
Other Names:
BTLA, B and T lymphocyte attenuator
Structure:
Ig superfamily, with similar structure to CTLA-4 and PD-1
Distribution:
B and T lymphocytes, NK cells and dendritic cells
Function:
Negative regulation of T cell activation, proliferation and cytokine production
Ligand Receptor:
HVEM
Antigen References:
1. Watanabe N, et al. 2003. Nat. Immunol. 4:670. 2. Sun Y, et al. 2009. J. Immunol. 183:1946. 3. Gonzalez LC, et al. 2005. Proc. Natl. Acad. Sci. USA 102:1116.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD272 (BTLA)
Human peripheral blood lymphocytes stained with CD3 (UCHT1) PerCP/Cy5.5 and purified MIH26 conjugated to PE (top) or mouse IgG2a,k PE isotype control
PE anti-human CD272 (BTLA)
Human peripheral blood lymphocytes stained with CD3 (UCHT1) PerCP/Cy5.5 and MIH26 PE (top) or mouse IgG2a,k PE isotype control
Biotin anti-human CD272 (BTLA)
Human peripheral blood lymphocytes stained with CD3 (UCHT1) APC and biotinylated MIH26 (top) or biotinylated mouse IgG2a, κ isotype control (bottom), followed by Sav-PE
APC anti-human CD272 (BTLA)
Human peripheral blood lymphocytes stained with CD19 (HIB19) PE and MIH26 APC (top) or mouse IgG2a, κ APC isotype control (bottom)
