Antibodies
|
Search   
Biotin anti-human CD258 (LIGHT) Antibody
Biotin anti-human CD258 (LIGHT) Antibody
318704 100 µg $195.00 Qty:
    

Product Details

 
Clone: T5-39 (See other available formats)
Isotype: Mouse IgG2b, κ
Isotype Control:Biotin Mouse IgG2b, κ Isotype Ctrl
Reactivity: Human
Immunogen: recombinant human LIGHT (TL5) protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Biotin anti-human CD258 (LIGHT) Antibody T5-39 Image 1
PMA/ionomycin-stimulated human peripheral blood lymphocytes (day-2) stained with biotinylated T5-39, followed by Sav-PE


Compare all formats



Cell Surface Immunofluorescence Staining Protocol




Description: The T5-39 antibody recognizes human LIGHT (CD258) also known as ligand for herpesvirus entry mediator, tumor necrosis factor receptor-like-2 ligand, TR2 ligand, and tumor necrosis factor ligand superfamily member 14 (TNFSF14). LIGHT is a type 2 transmembrane protein, and a member of the tumor necrosis factor ligand superfamily. LIGHT can be proteolytically cleaved and two isofoms have been shown to be produced by alternative splicing. The apparent molecular weight of LIGHT us approximately 30 kD. LIGHT expression has been observed in spleen, brain and kidney; expression is upregulated on activated T cells (particularly CD8+ cells). LIGHT stimulates proliferation of T cells and inhibits growth of some tumor cells. This protein has been shown to play a role in intestinal inflammation and contribute to IgA nephropathy. LIGHT is a ligand for HVEM, and LTβR and has also been shown to bind DcR3 and modulate function. In addition, LIGHT has been shown to interact with other proteins including TRAF2, TRAF3, and DIABLO. The T5-39 antibody has been shown to be useful for flow cytometry and ELISA.
Other Names: LIGHT, TL5, TNFSF14, TR2 ligand, Ligand for herpesvirus entry mediator, tumor necrosis factor receptor-like-2 ligand, tumor necrosis factor ligand superfamily member 14 (TNFSF14)
Structure: Type 2 transmembrane protein, member of the tumor necrosis factor ligand superfamily. Can be proteolytically cleaved; two isofoms produced by alternative splicing. Apparent molecular weight approximately 30 kD.
Distribution: Spleen, brain and kidney. Upregulated on activated T cells (particularly CD8+ cells)
Function: Stimulates proliferation of T cells, inhibits growth of some tumor cells. Has been shown to play a role in intestinal inflammation and contribute to IgA nephropathy
Ligand Receptor: Ligand for HVEM, LTβR. Has also been shown to bind DcR3
Interaction: TRAF2, TRAF3, DIABLO
Antigen
References:
1. Mauri DN, et al. 1998. Immunity 8:21.
2. Harrop JA, et al. 1998. J. Biol. Chem. 273:27548.
3. Morel Y, et al. 2000. J. Immunol. 165:4397.
4. Wang J, et al. 2004. J. Clin. Invest. 113:826.
5. Yu P, et al. 2004. Nature Immunol. 5:141.
GeneID: 8740
Latest Publications: View the latest CD258 articles on HighwirePress.com
UniProt: View information about CD258 on UniProt.org
Keywords: Biotin anti-human CD258 (LIGHT), T5-39, Biotin, LIGHT, TL5, TNFSF14, TR2 ligand, Ligand for herpesvirus entry mediator, tumor necrosis factor receptor-like-2 ligand, tumor necrosis factor ligand superfamily member 14 (TNFSF14), Human, Flow Cytometry, Immunology, Antibodies


Product Reviews

  Title Rating Date
- There are currently no reviews for this product. Be the first to review this product to qualify for a $100 Amazon gift card drawing. -
See more reviews using the Reviews Library


Product FAQs

 
How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
For a complete list of FAQs on all our products, visit our FAQ page.
 
Related Products

DescriptionCloneApplications
Biotin Mouse IgG2b, κ Isotype CtrlMPC-11FC, ICFC
APC StreptavidinFC, ICFC
PE StreptavidinFC, ICFC
PE/Cy5 StreptavidinFC, ICFC
PE/Cy7 StreptavidinFC, ICFC
Cell Staining BufferFC, ICC, ICFC
Human TruStain FcX™ (Fc Receptor Blocking Solution)FC, ICC, ICFC


Other Formats

DescriptionCloneApplications
PE anti-human CD258 (LIGHT)T5-39FC


Related Pathways

Pathway
Lymphotoxin-beta Receptor Pathway
TNF Superfamily Pathway


Related Categories

Category
Cell Biology > Antigens A-Z > LIGHT (CD258)
Immunology > Human Immunology > Cytokine and Chemokine Receptors > CD258 (LIGHT) > T5-39
Cytokines & Chemokines > Human Cytokine & Chemokine Research > Antibodies to Receptors > LIGHT (CD258) > T5-39
Immunology > Human Immunology > CD and Related Molecules > CD258 (LIGHT) > T5-39

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human CD258 (LIGHT)
    PMA/ionomycin-stimulated human peripheral blood lymphocytes

    PMA/ionomycin-stimulated human peripheral blood lymphocytes (day-2) stained with biotinylated T5-39, followed by Sav-PE

  • PE anti-human CD258 (LIGHT)
    PMA/ionomycin-stimulated (day-2) human peripheral blood

    PMA/ionomycin-stimulated (day-2) human peripheral blood lymphocytes stained with T5-39 PE

x
Copyright © 2014 BioLegend, Inc. All rights reserved.