Antibodies
      
 
Product Search  
 
Biotin anti-human CD258 (LIGHT) Antibody
Cat. # Size Price  

318704 100 µg $195.00
    
Product Details

Clone: T5-39 (See other available formats)
Isotype: Mouse IgG2b, κ
Isotype Control:Biotin Mouse IgG2b, κ Isotype Ctrl
Reactivity: Human
Immunogen: recombinant human LIGHT (TL5) protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
 Image 1
PMA/ionomycin-stimulated human peripheral blood lymphocytes (day-2) stained with biotinylated T5-39, followed by Sav-PE


Compare all formats





 


Antigen Details

Description: The T5-39 antibody recognizes human LIGHT (CD258) also known as ligand for herpesvirus entry mediator, tumor necrosis factor receptor-like-2 ligand, TR2 ligand, and tumor necrosis factor ligand superfamily member 14 (TNFSF14). LIGHT is a type 2 transmembrane protein, and a member of the tumor necrosis factor ligand superfamily. LIGHT can be proteolytically cleaved and two isofoms have been shown to be produced by alternative splicing. The apparent molecular weight of LIGHT us approximately 30 kD. LIGHT expression has been observed in spleen, brain and kidney; expression is upregulated on activated T cells (particularly CD8+ cells). LIGHT stimulates proliferation of T cells and inhibits growth of some tumor cells. This protein has been shown to play a role in intestinal inflammation and contribute to IgA nephropathy. LIGHT is a ligand for HVEM, and LTβR and has also been shown to bind DcR3 and modulate function. In addition, LIGHT has been shown to interact with other proteins including TRAF2, TRAF3, and DIABLO. The T5-39 antibody has been shown to be useful for flow cytometry and ELISA.
Other Names: LIGHT, TL5, TNFSF14, TR2 ligand, Ligand for herpesvirus entry mediator, tumor necrosis factor receptor-like-2 ligand, tumor necrosis factor ligand superfamily member 14 (TNFSF14)
Structure: Type 2 transmembrane protein, member of the tumor necrosis factor ligand superfamily. Can be proteolytically cleaved; two isofoms produced by alternative splicing. Apparent molecular weight approximately 30 kD.
Distribution: Spleen, brain and kidney. Upregulated on activated T cells (particularly CD8+ cells)
Function: Stimulates proliferation of T cells, inhibits growth of some tumor cells. Has been shown to play a role in intestinal inflammation and contribute to IgA nephropathy
Ligand Receptor: Ligand for HVEM, LTβR. Has also been shown to bind DcR3
Interaction: TRAF2, TRAF3, DIABLO
Antigen
References:
1. Mauri DN, et al. 1998. Immunity 8:21.
2. Harrop JA, et al. 1998. J. Biol. Chem. 273:27548.
3. Morel Y, et al. 2000. J. Immunol. 165:4397.
4. Wang J, et al. 2004. J. Clin. Invest. 113:826.
5. Yu P, et al. 2004. Nature Immunol. 5:141.
GeneID: 8740
UniProt: View information about CD258 on UniProt.org
Keywords: Biotin anti-human CD258 (LIGHT), T5-39, Biotin, LIGHT, TL5, TNFSF14, TR2 ligand, Ligand for herpesvirus entry mediator, tumor necrosis factor receptor-like-2 ligand, tumor necrosis factor ligand superfamily member 14 (TNFSF14), Human, Flow Cytometry, Immunology, Antibodies
Technical Data Sheet (pdf)
Material Safety Data Sheet
Certificate of Analysis
Request Bulk Quote
Request Custom Conjugation
Email Technical Support
Write a Review
View All CD258 Reagents
Related Protocols

Cell Surface Immunofluorescence Staining Protocol
Rating

This product has not been reviewed yet.
Related Pages

MojoSort™ Magnetic Cell Separation
Streptavidin Conjugates

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human CD258 (LIGHT)
    PMA/ionomycin-stimulated human peripheral blood lymphocytes

    PMA/ionomycin-stimulated human peripheral blood lymphocytes (day-2) stained with biotinylated T5-39, followed by Sav-PE

  • PE anti-human CD258 (LIGHT)
    PMA/ionomycin-stimulated (day-2) human peripheral blood

    PMA/ionomycin-stimulated (day-2) human peripheral blood lymphocytes stained with T5-39 PE

x
x
*Your Rating:
*Your Comment:
*Name (first, last):
*Affiliation:
*Email:
*Required fields
Note: Submission of a quick review does not qualify for our promotional $25 Amazon gift card. A full review submission is required for gift card eligibility.

Learn more…
9727 Pacific Heights Blvd.
San Diego, CA 92121

Tel: 858.768.5800
Company
Careers
Promotions
Web Tools
Areas of Biology
Pathways
Products
Literature
Custom Products/Services
Custom Panel Construction
Technical Support
Support Resources
Ordering
Contact
Distributors
Sitemap
Connect with us:

Facebook Twitter Pinterest Instagram LinkedIn YouTube Tumblr Blog Podcast
The LEGENDeers Pandora Google+ Wikipedia RSS Feed Arcade Words With Elements Satisfaction Survey
Privacy Policy | Terms
World-Class Quality | Superior Customer Support | Outstanding Value Copyright © 2014 BioLegend, Inc. All rights reserved.
Product Search