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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and/or by sandwich ELISA. For use as an ELISA detection antibody, a concentration range of 0.5-2 µg/ml is recommended. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308304) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). ELISA Detection: The biotinylated CARL-1 antibody is useful as the detection antibody in sandwich ELISA for measuring human TWEAK protein, in combination with CARL-2 antibody (Cat. No. 308402) used as ELISA capture. Neutralization1,2: In a dose-dependent manner, CARL-1 is more effective than CARL-21. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 308307).
Application References:
1. Nakayama, M., et al. 2000. J. Exp. Med. 192:1373. (Neut) 2. Nakayama, M., et al. 2003. J. Immunol. 170:341. (Neut)
Human TWEAK transfected cells stained with Carl-1 PE
TNF-like weak inducer of apoptosis (TWEAK) is a type II transmembrane TNF superfamily member with strong homology to TNF in its extracellular portion, though less potent in inducing apoptosis. It has been clustered as CD255. TWEAK transcript is expressed broadly in many adult and fetal tissues; however, the staining of human peripheral blood mononuclear cells with TWEAK-specific antibodies shows a more restricted pattern. TWEAK is expressed as membrane-bound and secreted forms. Interaction of TWEAK with its receptors (DR3, Fn14) promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, and apoptosis in a number of human cell lines.
Other Names:
TNF-like weak inducer of apoptosis, Apo-3 ligand (Apo3L), Death receptor 3-L (DR3-L), TNFSF 12, CD255
Structure:
TNF superfamily; 17 kD (Mammalian)
Distribution:
Normal tissue and tumor cells at low levels
Function:
Promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, apoptosis
Regulation:
Type II integral membrane protein readily cleaved from cell surface
Cellular Sources:
Transcripts widely expressed and abundant in most tissues; protein efficiently secreted
Cellular Targets:
Macrophages, astrocytes, endothelial cells
Receptors:
Apo-3 (DR3, WSL-1, TRAMP, LARD); TWEAKR
Bioactivity/Activities:
Weak apoptotic induction; mediate CD4+ T-cell killing of macrophages; induces IL-6, IL-8, ICAM-1 in astrocytes; HUVEC proliferation
Antigen References:
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Chicheportiche, Y., et al. 1997. J. Biol. Chem. 272:32401. 3. Kaplan, M., et al. 2000. J. Immunol. 164:2897. 4. Saas, P., et al. 2000. Glia 32:102. 5. Wiley, S., et al. 2001. Immunity 15:837.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD255 (TWEAK)
Human TWEAK transfected cells stained with Carl-1 PE
LEAF™ Purified anti-human CD255 (TWEAK)
Human TWEAK transfected cells stained with Carl-1 PE
PE anti-human CD255 (TWEAK)
Human TWEAK transfected cells stained with Carl-1 PE
Purified anti-human CD255 (TWEAK)
Human TWEAK transfected cells stained with Carl-1 PE
