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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemistry of formalin-fixed paraffin-embedded sections1 and acetone-fixed frozen sections, and blocking of CD200 interaction with CD200R.
Application References:
1. Palumbo GA, et al. 2009. Leuk Res. 33:1212. (IHC) 2. Foster-Cuevas M, et al. 2004. J. Virol. 78:7667. (FC)
Human peripheral blood lymphocytes stained with biotinylated OX-104, followed by Sav-PE
CD200, also known as OX2, is a member of the immunoglobulin superfamily (IgSF). It is a monomorphic cell surface glycoprotein that is expressed on thymocytes, neurons, endothelium, follicular dendritic cells in all lymphoid organs, a subset of CD34+ progenitor cells, and at low levels on some smooth muscle and B lymphocytes. It is not expressed on NK cells, monocytes, granulocytes, or platelets. CD200 costimulates T cell proliferation. It may regulate myeloid cell activity in a variety of tissues. The interaction between CD200 (OX2) and CD200 receptor (OX2R) system is of importance in the control of macrophage and granulocyte activation, which may contribute to pathways that suppress and limit macrophage induced inflammatory damage in tissue.
Other Names:
OX-2, OX2
Structure:
Immunoglobulin superfamily
Distribution:
T cells, neurons, endothelium
Function:
costimulates T cell proliferation
Regulation:
induces a downregulation of macrophage activity
Receptors:
CD200R
Antigen References:
1. Wright GJ, et al. 2001. Immunol. 102:173. 2. Foster-Cuevas M, et al. 2004. J. Virol. 78:7667. 3. Mason D, et al. 2002. ed. Leukocyte Typing VII. New York:Oxford Univ. Press. 4. Broderick C, et al. 2002. Am. J. Pathol. 161:1669.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD200 (OX2)
Human peripheral blood lymphocytes stained with purified OX-104, followed by anti-mouse IgG FITC
Biotin anti-human CD200 (OX2)
Human peripheral blood lymphocytes stained with biotinylated OX-104, followed by Sav-PE
PE anti-human CD200 (OX2)
Human peripheral blood lymphocytes stained with OX-104 PE
APC anti-human CD200 (OX2)
Human peripheral blood lymphocytes stained with OX-104 APC
