The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Application:
FC - Quality tested IHC* - *This application has been reported in the literature.
Recommended Usage:
This reagent is developed for immunofluorescent staining for flow cytometric analysis,
Application References:
1. McMichael, A., et al., 1987. Leucocyte Typing III. Oxford University Press. New York. 2. Knapp, W., et al., Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 3. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Human peripheral blood monocytes stained with biotinylated M5E2, followed by Sav-PE
Description:
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.
Other Names:
LPS receptor
Structure:
GPI-linked membrane glycoprotein, 53-55 kD
Distribution:
Monocytes, macrophages, granulocytes (low)
Function:
LPS receptor, clearance of Gram-negative pathogens
Ligand Receptor:
LPS
Antigen References:
1. Stocks, S., et al., 1990. Biochem. J. 268:275. 2. Wright, S., et al., 1990. Science 249:1434.
