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Biotin anti-human CD11a Antibody
Biotin anti-human CD11a Antibody
301204 100 µg $160.00 Qty:
    

Product Details

 
Clone: HI111 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Biotin Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
IV N231
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Capuchin Monkey, Horse (Equine), Cattle (Bovine, Cow), Sheep (Ovine), Dog (Canine), Rabbit (Lapine)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
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Application
Notes:

Clone HI111 epitope maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). HI111 is specific for the closed confirmation of the integrin.8 Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting2, and blocking of cell-cell interaction and inhibition the binding of ICAM-14. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301214).

Application
References:

  Publication Library
Biotin anti-human CD11a Antibody HI111 Image 1
Human peripheral blood lymphocytes stained with biotinylated HI111, followed by Sav-PE


Compare all formats



Cell Surface Immunofluorescence Staining Protocol




Description:

CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Other Names: LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL
Structure: Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β2 (CD18) forms LFA-1, 170-180 kD
Distribution: Leukocytes
Function: Adhesion, costimulation
Ligand Receptor: ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
Antigen
References:

1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146.

GeneID: 3683
Latest Publications: View the latest CD11a articles on HighwirePress.com
UniProt: View information about CD11a on UniProt.org
Keywords: Biotin anti-human CD11a, HI111, Biotin, LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL, Human, Cross-Reactivity: Chimpanzee, Baboon, Capuchin Monkey, Horse (Equine), Cattle (Bovine, Cow), Sheep (Ovine), Dog (Canine), Rabbit (Lapine), Flow Cytometry, Immunology, Antibodies


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Product FAQs

 
How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
For a complete list of FAQs on all our products, visit our FAQ page.
 
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Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-human CD11aHI111FC
Alexa Fluor® 594 anti-human CD11aHI111IF
Alexa Fluor® 647 anti-human CD11aHI111FC
APC anti-human CD11aHI111FC
FITC anti-human CD11aHI111FC
LEAF™ Purified anti-human CD11aHI111FC, Block, IHC, WB
PE anti-human CD11aHI111FC
PE/Cy5 anti-human CD11aHI111FC
PE/Cy7 anti-human CD11aHI111FC
Purified anti-human CD11aHI111FC, IHC, WB
Purified anti-human CD11a (MaxPar® Ready)HI111FC, CyTOF®


Related Pathways

Pathway
TH1 Pathway (Cellular Immune Response)
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Human Immunology > Myeloid Derived Suppressor Cells (MDSCs) > CD11a
Immunology > Human Immunology > Stem Cells > CD11a (integrin αL)
Immunology > Human Immunology > T Regulatory Cells (TReg) > CD11a (integrin αL)
Immunology > Human Immunology > Innate Immunity > CD11a (integrin αL)
Immunology > Non-Human Primate Immunology > CD11a (Integrin &alphaL)
Immunology > Human Immunology > Costimulatory Molecules > CD11a (integrin αL) > HI111
Immunology > Human Immunology > Adhesion Molecules > CD11a (integrin αL) > HI111
Immunology > Human Immunology > Th1/Th2 > CD11a (integrin αL) > HI111
Immunology > Human Immunology > CD and Related Molecules > CD11a (integrin αL) > HI111
Immunology > Human Immunology > Th17 > CD11a (integrin αL) > HI111
Cell Biology > Cell Adhesion > Integrin αL/CD11a

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 3 Revision Date: 2014-08-19
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 APC

  • Biotin anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated HI111, followed by Sav-PE

  • FITC anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 FITC

  • LEAF™ Purified anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified HI111, followed by anti-mouse IgGs FITC

  • PE anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 PE

  • PE/Cy5 anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 PE/Cy5

  • Purified anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified HI111, followed by anti-mouse IgGs FITC

  • Alexa Fluor® 488 anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 Alexa Fluor® 647

  • PE/Cy7 anti-human CD11a
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained CD11a (clone HI111) PE/Cy7 (filled histogram) or mouse IgG1, κ PE/Cy7 isotype control (open histogram).

  • Alexa Fluor® 594 anti-human CD11a
    Human neutrophils were fixed with

    Human neutrophils were fixed with 1% paraformaldehyde (PFA), then stained with 5 µg/ml anti-human CD11a (clone HI111) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

  • Purified anti-human CD11a (MaxPar® Ready)
    Human PBMCs stained with 154Sm-anti-CD45

    Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 142Nd-anti-CD11a (HI111). Data provided by DVS Sciences.

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