Mouse (recognizes all 3 forms), Rat (does not cross-react with human)
Immunogen:
Recombinant LIP
Formulation:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation:
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Application:
WB, IF
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Su, et al. 2003. J. Biol. Chem. 278:51150.
P388D1 (IL-1) cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-C/EBP ß antibody (Clone 1H7). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
Description:
C/EBP β (also known as CCAAT/enhancer-binding protein beta, interleukin 6-dependent DNA-binding protein, and nuclear factor of interleukin 6 [NF-IL6]) is a member of the basic leucine zipper C/EBP transcription factor family. C/EBP has three isoforms that include C/EBPβ, LAP, and LIP (35 kD, 32 kD, and 20 kD, respectively). This protein is localized in the nucleus and functions as a transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I gene expression. It is upregulated by growth hormones and has been shown to interact with CEBP-α, CEBP-δ, CEBP-γ, CEBP-ε, and CEBP-?. The C/EBP β protein can be modified by phosphorylation and ubiquitination. The 1H7 monoclonal antibody has been reported to be useful for Western blotting, immunoprecipitation, and immunofluorescence of mouse and rat C/EBP β, LIP, and LAP isoforms. The 1H7 antibody does not recognize human C/EBP β, LIP or LAP.
Other Names:
CCAAT/enhancer-binding protein beta, Alpha-1-acid glycoprotein/enhancer binding protein, Interleukin 6-dependent DNA-binding protein (IL-6DBP), Nuclear factor of interleukin 6 (NF-IL6), Liver-enriched transcriptional activator protein (LAP)
