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Biotin anti-Allophycocyanin (APC) Antibody
Biotin anti-Allophycocyanin (APC) Antibody
408003 25 µg $45.00     
408004 100 µg $100.00     
Product Details
Clone: APC003
Isotype: Mouse IgG1, κ
Immunogen: allophycocyanin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C. Do not freeze
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use at concentrations comparable to those of the primary antibody of interest. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
References:

1. Coppieters KT, et al. 2012. J. Exp Med. PubMed.

C57BL/6 thymocytes stained with RM4-5
C57BL/6 thymocytes stained with RM4-5 APC only (top), or with RM4-5 APC plus biotinylated APC003 (bottom), then detected with Sav-FITC




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Description:

The APC003 antibody reacts with both allophycocyanin (APC) alone and APC conjugated with antibody or streptavidin, as well as APC tandem dyes such as APC/Cy5.5 and APC/Cy7. When APC003 antibody is used in a three-step staining procedure or separation of APC-antibody labelled cells, it neither quenches APC fluorescence nor reacts with tested mouse and human cells. The APC003 antibody can be used for separation of APC-antibody labeled cells or as a secondary step to amplify APC conjugated primary antibody signals (i.e. biotinylated APC003 as secondary step, followed by Streptavidin-APC).
GeneID: NA
Latest Publications: View the latest Allophycocyanin articles on HighwirePress.com
UniProt: View information about Allophycocyanin on UniProt.org
Keywords: Biotin anti-Allophycocyanin (APC), APC003, Biotin, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Biotin anti-Allophycocyanin (APC)
    C57BL/6 thymocytes stained with RM4-5

    C57BL/6 thymocytes stained with RM4-5 APC only (top), or with RM4-5 APC plus biotinylated APC003 (bottom), then detected with Sav-FITC





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