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HRP Avidin
HRP Avidin
405103 1 ml £70     
Product Details
Clone: Avidin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.1% Proclin 150.
Storage & Handling: The Avidin-HRP solution should be stored undiluted at 4°C. Avoid exposure to sodium azide.
Application:

ELISA - Quality tested
ELISPOT, IHC, WB - Reported in the literature
Recommended Usage:

Each lot of this antibody is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:5000 to determine optimal conditions. For ELISPOT, dilute at 1:500 - 1:2500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
Notes:

Avidin-HRP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Av-HRP is recommended for ELISA, ELISPOT and Western blotting, when used with the relevant substrate system.



Description:

Avidin binds to biotin with high affinity. Avidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For Western blotting or ELISPOT applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.
Other Names: Avidin-Peroxidase, Avidin-Horseradish Peroxidase, Av-HRP
GeneID: NA
Latest Publications: View the latest Avidin articles on HighwirePress.com
UniProt: View information about Avidin on UniProt.org
Keywords: HRP Avidin, Avidin, HRP, Avidin-Peroxidase, Avidin-Horseradish Peroxidase, Av-HRP, Immunology, Antibodies
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