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APC/Cy7 Streptavidin
APC/Cy7 Streptavidin
405208 100 µg $175.00     

Product Details

Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated Streptavidin.
Concentration: 0.2 mg/ml
Storage & Handling: The Streptavidin APC/Cy7 solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested

Recommended Usage:

Each lot of this Streptavidin APC/Cy7 is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤0.06 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
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Application
Notes:

Streptavidin-Allophycocyanin/Cy7 (APC/Cy7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.

  Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application
References:

1. Maresz K, et al. 2005. J. Neurochemistry 95:437.
2. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.J. Immunol.
3. Ponomarev ED, et al. 2007. J. Immunol. 178:39.
4. Mann MK, et al. 2007. J. Immunol. 178:3447.PubMed
5. Ponomarev ED,et al.2005.J. Immunol Methods.300:32. PubMed
6. Cao YA, et al. 2008. BloodPubMed
7. Garidou L, et al. 2009. J. Virol. PubMed
8. Dolgachev V, et al. 2009. J. Immunol. 183:5705. PubMed




Description:

Streptavidin binds to biotin with high affinity. Streptavidin-APC/Cy7 is useful for detecting biotinylated antibodies. The excitation of APC/Cy7 by 600-635 nm laser light induces a fluorescence emission maximum of 780 nm.

Other Names: Streptavidin-Allophycocyanin/Cy7, SAv-APC/Cy7
GeneID: NA
Latest Publications: View the latest Streptavidin articles on HighwirePress.com
UniProt: View information about Streptavidin on UniProt.org
Keywords: APC/Cy7 Streptavidin, APC/Cy7, Streptavidin-Allophycocyanin/Cy7, SAv-APC/Cy7, Mouse, Human, Rat, Immunology, Antibodies
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