Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The B20.1 antibody recognizes most members of the Vα2 TCR subfamily in mice having the a, b, and c haplotypes.
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Pircher H, et al. 1992. Eur. J. Immunol.. 22:399. 2. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077.
C57BL/6 mouse splenocytes were stained with CD3 PE and anti-mouse TCR Vα2 (clone B20.1) APC/Cy7 (top) or rat IgG2a APC/Cy7 isotype control (bottom).
The TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ). TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.
Other Names:
T cell receptor Vα 2
Distribution:
On a subset of T cells
Function:
In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
Ligand Receptor:
TCR recognizes MHC/peptide complex
Interaction:
Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
Antigen References:
1. Kubo RT, et al. 1989. J. Immunol. 142:2736. 2. Pircher H, et al. 1992. Eur. J. Immunol.. 22:399.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse TCR Vα2
C57BL/6 splenocytes stained with CD3 (145-2C11) APC and purified B20.1, followed by anti-rat IgG PE
Biotin anti-mouse TCR Vα2
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and biotinylated B20.1, followed by Sav-PE
FITC anti-mouse TCR Vα2
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and B20.1 FITC
PE anti-mouse TCR Vα2
C57BL/6 splenocytes stained with 145-2C11 (CD3) APC and B20.1 PE
APC anti-mouse TCR Vα2
C57BL/6 splenocytes stained with 145-2C11 (CD3) PE and B20.1 APC
Alexa Fluor® 647 anti-mouse TCR Vα2
BALB/c splenocytes stained with B20.1 Alexa Fluo® 647
PerCP/Cy5.5 anti-mouse TCR Vα2
BALB/c splenocytes stained with B20.1 PerCP/Cy5.5
Pacific Blue™ anti-mouse TCR Vα2
C57BL/6 mouse splenocytes stained with B20.1 Pacific Blue™ and CD3e PE
C57BL/6 mouse splenocytes stained with rat IgG2a, λ Pacific Blue™ isotype control and CD3e PE
APC/Cy7 anti-mouse TCR Vα2
C57BL/6 mouse splenocytes were stained with CD3 PE and anti-mouse TCR Vα2 (clone B20.1) APC/Cy7 (top) or rat IgG2a APC/Cy7 isotype control (bottom).
