The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemistry6,7 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 103014).
Application References:
1. Trowbridge, I.S., et al. 1982. Immunogenetics 15:299. (ICFC, IP, C') 2. Katoh, S., et al. 1994. J. Immunol. 153:3440. (ELISA) 3. Budd, R.C., et al. 1987. J. Immunol. 138:3120. (IP) 4. Camp, R.L., et al. 1993. J. Exp. Med. 178:497. (Block) 5. Weiss, J.M., et al. 1997. J. Cell Biol. 137:1137. (Block) 6. Frank, N.Y., et al. 2005. Cancer Res. 65:4320. (IHC) PubMed 7. Cuff, C.A., et al. 2001. J. Clin. Invest. 108:1031. (IHC) 8. Lee, J-W., et al. 2006. Nature Immunol. 8:181. 9. Zhang, N., et al. 2005..J. Immunol. 174:6967. PubMed 10. Huabiao, C., et al. 2005. J. Immunol. 175:591.PubMed 11. Gui, J., et al. 2007. Int Immunol. 19:1201. PubMed 12. Wang, X.Y., et al.2008.Blood.111:2436. PubMed 13. Kenna, T.J., et al.2008. Blood.111:2091.PubMed 14. Yamazaki, J., et al. 2009. Blood. PubMed 15. Kmieciak, M., et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
C57BL/6 splenocytes stained with IM7 APC/Cy7
Description:
CD44 is a 80-95 kD glycoprotein, also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from a low or mid level intensity to high expression levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to, and rolling on, endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation. Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44.
Other Names:
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Structure:
Variable splicing of CD44 gene generates many CD44 isoforms, 85 kD
Distribution:
All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
Function:
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand Receptor:
Hyaluronan, MIP-1ß, fibronectin, collagen
Antigen References:
1. Barclay, A.N., et al. 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Haynes, B.F., et al. 1991. Cancer Cells 3:347. 3. Goldstein, L.A., et al. 1989. Cell 56:1063. 4. Mikecz, K., et al. 1995. Nat. Med. 1:558. 5. Hegde, V., et al. 2008. J. Leukoc. Biol. 84:134. 6. Liu, T., et al. 2009. Biol. Direct 4:40.
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