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The antibody was purified by affinity chromatography and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections. The HIT8a antibody does not block the binding of RPA-T8 antibody to CD8a.
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. 3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
Human peripheral blood lymphocytes stained with HIT8a APC/Cy7
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Other Names:
T8, Leu2
Structure:
Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution:
Majority of thymocytes, T cell subset, NK cells
Function:
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand Receptor:
MHC Class I molecules
Antigen References:
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD8a
Human peripheral blood lymphocytes stained
with HIT8a FITC
Biotin anti-human CD8a
Human peripheral blood lymphocytes stained with biotinylated HIT8a, followed by Sav-PE
FITC anti-human CD8a
Human peripheral blood lymphocytes stained
with HIT8a FITC
PE anti-human CD8a
Human peripheral blood lymphocytes stained with HIT8a PE
PE/Cy5 anti-human CD8a
Human peripheral blood lymphocytes stained
with HIT8a FITC
Purified anti-human CD8a
Human peripheral blood lymphocytes stained with purified HIT8a, followed by anti - mouse IgGs FITC
PE/Cy7 anti-human CD8
Human peripheral blood lymphocytes stained with HIT8a PE/Cy7
Alexa Fluor® 488 anti-human CD8a
Human peripheral blood lymphocytes stained with HIT8a Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD8a
Human peripheral blood lymphocytes stained with HIT8a Alexa Fluor® 647
Alexa Fluor® 700 anti-human CD8a
Human peripheral blood lymphocytes stained with HIT8a Alexa Fluor® 700
PerCP anti-human CD8a
Human peripheral blood lymphocytes stained with HIT8a PerCP
PerCP/Cy5.5 anti-human CD8a
Human peripheral blood lymphocytes stained with HIT8a PerCP/Cy5.5
APC/Cy7 anti-human CD8a
Human peripheral blood lymphocytes stained with HIT8a APC/Cy7
Pacific Blue™ anti-human CD8a
Human peripheral blood lymphocytes stained with HIT8a Pacific Blue™
