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The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Kan EA, et al. 1983. J. Immunol. 131:536. 2. Wood GS, et al. 1985. Am. J. Pathol. 120:371. 3. Van Dongen JJM, et al. 1988. Blood 71:603. (WB) 4. Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC) 5. Carbone A, et al. 1999. Blood 93:2319. (IHC) 6. Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF) 7. Rutjens E, et al. 2007. J. Immunol. 178:1702. 8. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC) 9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes stained with SK7 APC/Cy7
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Other Names:
T3, CD3ε
Structure:
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution:
Mature T and NK-T cells, thymocyte differentiation
Function:
Antigen recognition, signal transduction, T cell activation
Ligand Receptor:
Peptide antigen bound to MHC
Antigen References:
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD3
Human peripheral blood lymphocytes stained with biotinylated anti-human CD3 (clone SK7, filled histogram) or biotinylated mouse IgG1, κ (open histogram) isotype control, followed by SAV-PE
Purified anti-human CD3
Human peripheral blood lymphocytes stained with SK7 FITC.
FITC anti-human CD3
Human peripheral blood lymphocytes stained with SK7 FITC.
PE anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (clone SK7) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Alexa Fluor® 488 anti-human CD3
Human peripheral blood lymphocytes stained with SK7 Alexa Fluor® 488
APC anti-human CD3
Human peripheral blood lymphocytes stained with SK7 APC
PerCP/Cy5.5 anti-human CD3
Human peripheral blood lymphocytes stained with SK7 PerCP/Cy5.5
PerCP anti-human CD3
Human peripheral blood lymphocytes stained with SK7 PerCP
PE/Cy7 anti-human CD3
Human peripheral blood lymphocytes stained with SK7 PE/Cy7
APC/Cy7 anti-human CD3
Human peripheral blood lymphocytes stained with SK7 APC/Cy7
