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APC/Cy7 anti-human CD3 Antibody
APC/Cy7 anti-human CD3 Antibody
300317 25 tests $130.00 Qty:
    
300318 100 tests $265.00 Qty:
    

Product Details

 
Clone: HIT3a (See other available formats)
Isotype: Mouse IgG2a, κ
Isotype Control:APC/Cy7 Mouse IgG2a, κ Isotype Ctrl
Workshop
Number:
V CD03.05
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Storage & Handling: The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.

Cy® and CyDye® are registered trademarks of GE Healthcare.

Excitation
Laser:
Red Laser (633 nm)
COA:
Enter Lot#:   
Application
Notes:

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application
References:

  Publication Library
APC/Cy7 anti-human CD3 Antibody HIT3a Image 1
Human peripheral blood lymphocytes stained with HIT3a APC/Cy7


Compare all formats

See APC/Cy7 spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Other Names: T3, CD3ε
Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK-T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand Receptor: Peptide antigen bound to MHC
Antigen
References:

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

GeneID: 916
Latest Publications: View the latest CD3 articles on HighwirePress.com
UniProt: View information about CD3 on UniProt.org
Keywords: APC/Cy7 anti-human CD3, HIT3a, APC/Cy7, T3, CD3ε, Human, Flow Cytometry, Immunology, Antibodies


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Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-human CD3HIT3aFC
Alexa Fluor® 647 anti-human CD3HIT3aFC
Alexa Fluor® 700 anti-human CD3HIT3aFC
APC anti-human CD3HIT3aFC
Biotin anti-human CD3HIT3aFC
FITC anti-human CD3HIT3aFC
LEAF™ Purified anti-human CD3HIT3aFC, IP, Activ, IHC
Pacific Blue™ anti-human CD3HIT3aFC
PE anti-human CD3HIT3aFC
PE/Cy5 anti-human CD3HIT3aFC
PE/Cy7 anti-human CD3HIT3aFC
PerCP/Cy5.5 anti-human CD3HIT3aFC
Purified anti-human CD3HIT3aFC, IHC, IP
Ultra-LEAF™ Purified anti-human CD3HIT3aFC, IP, Activ, IHC


Related Pathways

Pathway
CD28 Signaling in T-Helper Cell
CD4 and CD8 T-Cell Lineage
ERK Signaling
Gamma-Delta T cells
Immunologic Networks 2011
Innate Immunity
MDSC-induced Immune Suppression
NFAT Signaling and Lymphocyte Interactions
TH1 Pathway (Cellular Immune Response)
Th17 Biology
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Non-Human Primate Immunology > CD3
Immunology > Human Immunology > T Regulatory Cells (TReg) > CD3 (T3)
Immunology > Human Immunology > T Cell Receptors (TCRs) > CD3 > HIT3a
Immunology > Human Immunology > CD and Related Molecules > CD3 (T3) > HIT3a
Immunology > Human Immunology > Costimulatory Molecules > CD3 > HIT3a
Immunology > Human Immunology > Innate Immunity > CD3 > HIT3a
CD3 > HIT3a
HIT3a
HIT3a
Immunology > Human Immunology > Stem Cells > CD3 (T3) > HIT3a
Immunology > Human Immunology > NK/NKT > CD3 > HIT3a
Immunology > Human Immunology > Th1/Th2 > CD3 > HIT3a
Immunology > Human Immunology > Th17 > CD3 > HIT3a

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT3a APC

  • Biotin anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated HIT3a, followed by Sav-PE

  • FITC anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT3a FITC

  • LEAF™ Purified anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAFTM purified HIT3a and detected with anti-mouse IgGs FITC

  • PE anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT3a PE

  • PE/Cy5 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT3a PE/Cy5

  • Purified anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified HIT3a and detected with anti-mouse IgGs FITC

  • APC/Cy7 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT3a APC/Cy7

  • PE/Cy7 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT3a PE/Cy7

  • Alexa Fluor® 488 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT3a Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT3a Alexa Fluor® 647

  • Alexa Fluor® 700 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT3a Alexa Fluor® 700

  • PerCP/Cy5.5 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT3a PerCP/Cy5.5

  • Pacific Blue™ anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT3a Pacific Blue™

  • Ultra-LEAF™ Purified anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified HIT3a and detected with anti-mouse IgGs FITC

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