Antibodies
|
Search   
APC/Cy7 anti-human CD20 Antibody
APC/Cy7 anti-human CD20 Antibody
302313 25 tests $130.00 Qty:
    
302314 100 tests $265.00 Qty:
    

Product Details

 
Clone: 2H7 (See other available formats)
Isotype: Mouse IgG2b, κ
Isotype Control:APC/Cy7 Mouse IgG2b, κ Isotype Ctrl
Workshop
Number:
IV B201
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey
Immunogen: Human tonsillar B cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.

Cy® and CyDye® are registered trademarks of GE Healthcare.

Excitation
Laser:
Red Laser (633 nm)
COA:
Enter Lot#:   
Application
Notes:

The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.

Application
References:

  Publication Library
APC/Cy7 anti-human CD20 Antibody 2H7 Image 1
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) APC/Cy7 (filled histogram), or mouse IgG2b, κ APC/Cy7 (dashed line).


Compare all formats

See APC/Cy7 spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Other Names: B1, Bp35
Structure: Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution: B cell, T cell subsets
Function: B cell activation
Ligand Receptor: Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Antigen
References:

1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.

GeneID: 931
Latest Publications: View the latest CD20 articles on HighwirePress.com
UniProt: View information about CD20 on UniProt.org
Keywords: APC/Cy7 anti-human CD20, 2H7, APC/Cy7, B1, Bp35, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Flow Cytometry, Immunology, Antibodies


Product Reviews

  Title Rating Date
- There are currently no reviews for this product. Be the first to review this product to qualify for a $100 Amazon gift card drawing. -
See more reviews using the Reviews Library


Product FAQs

 
What is the difference between two anti human CD20 clones 2H7 and 1412?
For clone 1412: This clone specifically recognizes cytoplasmic domain of CD20 and thus can only be used for intracellular flow cytometry. In this instance you will need to include the fixation and permeabilization steps. Please follow the protocol described below.

http://www.biolegend.com/media_assets/support_protocol/Biolegend_Intracell_Cytok_Stain_Protocol_121511.pdf

For clone 2H7: This clone is ok for regular surface staining for CD20 and there is no need for any fixation and permeabilization steps. Below is the link for the relevant protocol.

http://www.biolegend.com/media_assets/support_protocol/BioLegend_Surface_Staining_Flow_Protocol_121511.pdf
For a complete list of FAQs on all our products, visit our FAQ page.
 
Related Products

DescriptionCloneApplications
APC anti-human CD3UCHT1FC
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC
APC/Cy7 anti-human CD19HIB19FC
APC/Cy7 anti-human CD3HIT3aFC
APC/Cy7 Mouse IgG2b, κ Isotype CtrlMPC-11FC, ICFC
Human TruStain FcX™ (Fc Receptor Blocking Solution)FC, ICC, ICFC


Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-human CD202H7FC
Alexa Fluor® 647 anti-human CD202H7FC
Alexa Fluor® 700 anti-human CD202H7FC
APC anti-human CD202H7FC
Brilliant Violet 421™ anti-human CD202H7FC
Brilliant Violet 510™ anti-human CD202H7FC
Brilliant Violet 570™ anti-human CD202H7FC
Brilliant Violet 605™ anti-human CD202H7FC
Brilliant Violet 650™ anti-human CD202H7FC
Brilliant Violet 711™ anti-human CD202H7FC
FITC anti-human CD202H7FC
Pacific Blue™ anti-human CD202H7FC
PE anti-human CD202H7FC
PE/Cy5 anti-human CD202H7FC
PE/Cy7 anti-human CD202H7FC
PE/Dazzle™ 594 anti-human CD202H7FC
PerCP anti-human CD202H7FC
PerCP/Cy5.5 anti-human CD202H7FC
Purified anti-human CD202H7FC, IHC, IP, CyTOF®
Purified anti-human CD20 (MaxPar® Ready)2H7FC, CyTOF®


Related Pathways

Pathway
B cell Development and Activation


Related Categories

Category
2H7
Immunology > Human Immunology > CD and Related Molecules > CD20 (Bp35, B1) > 2H7
Immunology > Human Immunology > Stem Cells > CD20 (Bp35, B1) > 2H7
Immunology > Human Immunology > Costimulatory Molecules > CD20 (Bp35, B1) > 2H7
Immunology > Human Immunology > B Cells > CD20 (Bp35, B1) > 2H7
Immunology > Non-Human Primate Immunology > CD20

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 3 Revision Date: 2013-10-08
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) APC (filled histogram), or mouse IgG2b, κ APC (open histogram).

  • FITC anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) FITC (dark line), or mouse IgG2b, κ FITC (dashed line).

  • PE anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PE (solid line), or mouse IgG2b, κ PE (dashed line).

  • PE/Cy5 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PE/Cy5 (solid line), or mouse IgG2b, κ PE/Cy5 (dashed line).

  • Purified anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with purified anti-CD20 (clone 2H7) (solid line), or mouse IgG2b, κ (dashed line) followed by anti-mouse IgGs FITC.

  • APC/Cy7 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) APC/Cy7 (filled histogram), or mouse IgG2b, κ APC/Cy7 (dashed line).

  • PE/Cy7 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PE/Cy7 (solid line), or mouse IgG2b, κ PE/Cy7 (dashed line).

  • Alexa Fluor® 488 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 488 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 488 (open histogram).

  • Alexa Fluor® 647 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 647 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 647 (open histogram).

  • Pacific Blue™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Pacific Blue™ (filled histogram), or mouse IgG2b, κ Pacific Blue™ (open histogram).

  • Alexa Fluor® 700 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 700 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 700 (open histogram).

  • PerCP anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PerCP (filled histogram), or mouse IgG2b, κ PerCP (open histogram).

  • PerCP/Cy5.5 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PerCP/Cy5.5 (filled histogram), or mouse IgG2b, κ PerCP/Cy5.5 (open histogram).

  • Brilliant Violet 421™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD19 PE and CD20 (clone 2H7) Brilliant Violet 421™ (top) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom).





  • Brilliant Violet 570™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 570™ (filled histogram), or mouse IgG2b, κ Brilliant Violet 570™ (dashed line).

  • Brilliant Violet 605™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 605™.

  • Brilliant Violet 650™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 650™.

  • Brilliant Violet 510™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 510™ (filled histogram) or mouse IgG2b, κ Brilliant Violet 510™ (open histogram).

  • Brilliant Violet 711™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 711™ (filled histogram). Open histogram represents unstained cells.

  • Purified anti-human CD20 (MaxPar® Ready)
    Human PBMCs stained with 154Sm-anti-CD45

    Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 147m-anti-CD20 (2H7). Data provided by DVS Sciences.

  • PE/Dazzle™ 594 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 PE/Dazzle™ 594 (clone 2H7) (filled histogram) or Mouse IgG2b, κ PE/Dazzle™ 594 (open histogram).

x
Copyright © 2014 BioLegend, Inc. All rights reserved.  
?>