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APC/Cy7 anti-human CD20 Antibody
Cat. # Size Price  

302313 25 tests $130.00
    
302314 100 tests $265.00
    
Product Details

Clone: 2H7 (See other available formats)
Isotype: Mouse IgG2b, κ
Isotype Control:APC/Cy7 Mouse IgG2b, κ Isotype Ctrl
Workshop
Number:
IV B201
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey
Immunogen: Human tonsillar B cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.Cy® and CyDye® are registered trademarks of GE Healthcare.
Excitation
Laser:
Red Laser (633 nm)
Application
Notes:
The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.
Application
References:
  Publication Library
 Image 1
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) APC/Cy7 (filled histogram), or mouse IgG2b, κ APC/Cy7 (dashed line).


Compare all formats

See APC/Cy7 spectral data





 


Antigen Details

Description: CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
Other Names: B1, Bp35
Structure: Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution: B cell, T cell subsets
Function: B cell activation
Ligand Receptor: Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Antigen
References:
1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.
GeneID: 931
UniProt: View information about CD20 on UniProt.org
Keywords: APC/Cy7 anti-human CD20, 2H7, APC/Cy7, B1, Bp35, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Flow Cytometry, Immunology, Antibodies
Technical Data Sheet (pdf)
Material Safety Data Sheet
Certificate of Analysis
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Related Protocols

Cell Surface Immunofluorescence Staining Protocol
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Version: 3 Revision Date: 2013-10-08
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) APC (filled histogram), or mouse IgG2b, κ APC (open histogram).

  • FITC anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) FITC (dark line), or mouse IgG2b, κ FITC (dashed line).

  • PE anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PE (solid line), or mouse IgG2b, κ PE (dashed line).

  • PE/Cy5 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PE/Cy5 (solid line), or mouse IgG2b, κ PE/Cy5 (dashed line).

  • Purified anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with purified anti-CD20 (clone 2H7) (solid line), or mouse IgG2b, κ (dashed line) followed by anti-mouse IgGs FITC.

  • APC/Cy7 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) APC/Cy7 (filled histogram), or mouse IgG2b, κ APC/Cy7 (dashed line).

  • PE/Cy7 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PE/Cy7 (solid line), or mouse IgG2b, κ PE/Cy7 (dashed line).

  • Alexa Fluor® 488 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 488 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 488 (open histogram).

  • Alexa Fluor® 647 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 647 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 647 (open histogram).

  • Pacific Blue™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Pacific Blue™ (filled histogram), or mouse IgG2b, κ Pacific Blue™ (open histogram).

  • Alexa Fluor® 700 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 700 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 700 (open histogram).

  • PerCP anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PerCP (filled histogram), or mouse IgG2b, κ PerCP (open histogram).

  • PerCP/Cy5.5 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PerCP/Cy5.5 (filled histogram), or mouse IgG2b, κ PerCP/Cy5.5 (open histogram).

  • Brilliant Violet 421™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD19 PE and CD20 (clone 2H7) Brilliant Violet 421™ (top) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom).





  • Brilliant Violet 570™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 570™ (filled histogram), or mouse IgG2b, κ Brilliant Violet 570™ (dashed line).

  • Brilliant Violet 605™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 605™.

  • Brilliant Violet 650™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 650™.

  • Brilliant Violet 510™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 510™ (filled histogram) or mouse IgG2b, κ Brilliant Violet 510™ (open histogram).

  • Brilliant Violet 711™ anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 711™ (filled histogram). Open histogram represents unstained cells.

  • Purified anti-human CD20 (MaxPar® Ready)
    Human PBMCs stained with 154Sm-anti-CD45

    Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 147m-anti-CD20 (2H7). Data provided by DVS Sciences.

  • PE/Dazzle™ 594 anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD20 PE/Dazzle™ 594 (clone 2H7) (filled histogram) or Mouse IgG2b, κ PE/Dazzle™ 594 (open histogram).

  • Biotin anti-human CD20
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with biotinylated CD20 (clone 2H7) (filled histogram) or biotinylated mouse IgG2b, κ isotype control (open histogram), followed by Sav-PE.

  • Alexa Fluor® 594 anti-human CD20
    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA) then blocked with 5% FBS. The cells were then stained with 10 µg/ml CD20 (clone 2H7) Alexa Fluor® 594 (red), CD3 (clone UCHT1) Alexa Fluor® 647 (yellow), and CD14 (clone HCD14) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

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