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The antibody was purified by affinity chromatography, and conjugated with APC/Cy5.5 under optimal conditions. The solution is free of unconjugated APC/Cy5.5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, and immunoprecipitation1,2. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody (Cat. No. 100506), but not RM4-4 antibody (Cat. No. 116002). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 100416). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP) 2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete) 3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim) 4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block) 5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete) 6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete) 7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete) 8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete) 9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC) 10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC) 11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) APC/Cy5.5 (filled histogram) or rat IgG2b, κ APC/Cy5.5 isotype control (open histogram).
CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.
Other Names:
L3T4, T4
Structure:
Ig superfamily, 55 kD
Distribution:
Majority of thymocytes, T cell subset
Function:
TCR co-receptor, T cell activation
Ligand Receptor:
MHC class II molecule
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579. 3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
Biotin anti-mouse CD4
C57BL/6 mouse splenocytes were stained with biotinylated CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE.
FITC anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) FITC (filled histogram) or rat IgG2b, κ FITC isotype control (open histogram).
LEAF™ Purified anti-mouse CD4
C57BL/6 mouse splenocytes were stained with LEAF™ purified CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC.
PE anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram).
PE/Cy5 anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE/Cy5 (filled histogram) or rat IgG2b, κ PE/Cy5 isotype control (open histogram).
Purified anti-mouse CD4
BALB/c mouse splenocytes were stained with purified CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC.
PE/Cy7 anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE/Cy7 (filled histogram) or rat IgG2b, κ PE/Cy7 isotype control (open histogram).
APC/Cy5.5 anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) APC/Cy5.5 (filled histogram) or rat IgG2b, κ APC/Cy5.5 isotype control (open histogram).
APC/Cy7 anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) APC/Cy7 (filled histogram) or rat IgG2b, κ APC/Cy7 isotype control (open histogram).
Alexa Fluor® 647 anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) Alexa Fluor® 647 (filled histogram) or rat IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
Alexa Fluor® 488 anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) Alexa Fluor® 488 (solid line) or rat IgG2b, κ Alexa Fluor® 488 isotype control (broken line).
Pacific Blue™ anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) Pacfic Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram).
Alexa Fluor® 700 anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone GK1.5) Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram).
PerCP anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PerCP (filled histogram) or rat IgG2b, κ PerCP isotype control (open histogram).
PerCP/Cy5.5 anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PerCP/Cy5.5 (filled histogram) or rat IgG2b, κ PerCP/Cy5.5 isotype control (open histogram).
Brilliant Violet 421™ anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD4 (clone GK1.5) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
Ultra-LEAF™ Purified anti-mouse CD4
C57BL/6 mouse splenocytes were stained with LEAF™ purified CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC.
