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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking1 of allogeneic responses, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 200612).
Application References:
1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. 2. Matta B, et al. 2008. Am J Pathol. 173:1440. PubMed
CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
Other Names:
CD8a, T8, Leu-8, Ly-2, Lyt2
Structure:
Ig superfamily, CD8α chain
Distribution:
Most thymocytes, T cell subset; activated CD4+ T cells (not resting), most NK cells and intestinal intraepithelial cells express the CD8α chain without the CD8β chain
Function:
Co-receptor for TCR
Ligand Receptor:
MHC class I molecule
Antigen References:
1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609. 2. Dallman MJ, et al. 1982. Eur. J. Immunol. 12:511. 3. Cantrell DA, et al. 1982. Immunol. 45:97. 4. Lyscom N, et al. 1982. Immunol. 45:775. 5. Johnson P, et al. 1985. EMBO. J. 4:2539. 6. Barclay AN. 1981. Immunol. 42:593.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-rat CD8a
LOU rat splenocytes stained with G28 APC
Biotin anti-rat CD8a
LOU rat splenocytes stained with G28 biotin, followed by SAV-PE
PE anti-rat CD8a
LOU rat splenocytes stained with G28 PE
Purified anti-rat CD8a
Rat splenocytes stained with purified G28, followed by anti-mouse IgG FITC
