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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The JJ319 antibody has been reported to augment in vitro T cell proliferation and enhance IL-2 production. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vitro costimulation of proliferation responses1, and in vitro costimulation of IL-2 production in the presence of R73 antibody (anti-rat α/β TCR, Cat. No. 201112)2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 200910).
Application References:
1. Tacke M, et al. 1995. J. Immunol. 154:5121. (Costim) 2. Tacke M, et al. 1997. Eur. J. Immunol. 27:239. (Costim)
CD28 is a 44 kD glycoprotein also known as Tp44 or T44. It is a member of the immunoglobulin superfamily and is expressed on thymocytes, α/βTCR+ T cells, most γ/δTCR+ T cells, and an NK cell subset. During interaction with CD80 (B7-1) or CD86 (B7-2), CD28 acts as the second signal for T cell stimulation.
Other Names:
Tp44, T44
Structure:
Ig superfamily, 44 kD
Distribution:
All α/βTCR+ T cells, most γ/δTCR+ T cells, NK cell subset
Function:
T cell costimulation
Ligand Receptor:
CD80 (B7-1) and CD86 (B7-2)
Antigen References:
1. Tacke M, et al. 1995. J. Immunol. 154:5121. 2. Mitnancht R, et al. 1995. Eur. J. Immunol. 25:328. 3. Tacke M, et al. 1997. Eur. J. Immunol. 27:239.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-rat CD28
LOU rat splenocytes stained with JJ319 APC
FITC anti-rat CD28
LOU rat splenocytes stained with JJ319 FITC
Purified anti-rat CD28
LOU rat splenocytes stained with purified JJ319, followed by anti-mouse IgG FITC
