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APC anti-mouse TCR Vα2 Antibody
APC anti-mouse TCR Vα2 Antibody
127809 25 µg $95.00     
127810 100 µg $195.00     
Product Details
Clone: B20.1
Isotype: Rat IgG2a, λ
Reactivity: Mouse
Immunogen: Soluble TCR from mouse CTL clone KB5-C20
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
Notes:

The B20.1 antibody recognizes most members of the Vα2 TCR subfamily in mice having the a, b, and c haplotypes.
Application
References:

1. Pircher H, et al. 1992. Eur. J. Immunol.. 22:399.
2. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077.
3. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed
4. Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed
C57BL/6 splenocytes stained with 145-2C11
C57BL/6 splenocytes stained with 145-2C11 (CD3) PE and B20.1 APC


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Description:

The TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.
Other Names: T cell receptor Vα 2
Distribution: On a subset of T cells
Function: In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
Ligand Receptor: TCR recognizes MHC/peptide complex
Interaction: Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
Antigen
References:

1. Kubo RT, et al. 1989. J. Immunol. 142:2736
2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399
GeneID: 21473
Latest Publications: View the latest TCR V alpha 2 articles on HighwirePress.com
UniProt: View information about TCR V alpha 2 on UniProt.org
Keywords: APC anti-mouse TCR Vα2, B20.1, APC, T cell receptor Vα 2 , Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-mouse TCR Vα2
    C57BL/6 splenocytes stained with CD3

    C57BL/6 splenocytes stained with CD3 (145-2C11) APC and purified B20.1, followed by anti-rat IgG PE

  • Biotin anti-mouse TCR Vα2
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and biotinylated B20.1, followed by Sav-PE

  • FITC anti-mouse TCR Vα2
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and B20.1 FITC

  • PE anti-mouse TCR Vα2
    C57BL/6 splenocytes stained with 145-2C11

    C57BL/6 splenocytes stained with 145-2C11 (CD3) APC and B20.1 PE

  • APC anti-mouse TCR Vα2
    C57BL/6 splenocytes stained with 145-2C11

    C57BL/6 splenocytes stained with 145-2C11 (CD3) PE and B20.1 APC

  • Alexa Fluor® 647 anti-mouse TCR Vα2
    BALB/c splenocytes stained with B20.1

    BALB/c splenocytes stained with B20.1 Alexa Fluo® 647

  • PerCP/Cy5.5 anti-mouse TCR Vα2
    BALB/c splenocytes stained with B20.1

    BALB/c splenocytes stained with B20.1 PerCP/Cy5.5

  • Pacific Blue™ anti-mouse TCR Vα2
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with B20.1 Pacific Blue™ and CD3e PE

    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with rat IgG2a, λ Pacific Blue™ isotype control and CD3e PE

  • APC/Cy7 anti-mouse TCR Vα2
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD3 PE and anti-mouse TCR Vα2 (clone B20.1) APC/Cy7 (top) or rat IgG2a APC/Cy7 isotype control (bottom).





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