The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
Moriyama Y et al. 2008. Int J Immunology 20:763
Mouse Notch 2 transfected cells stained with HMN2-35 APC
Description:
The Notch receptors are highly conserved from invertebrates to mammals.Notch 1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors.The Notch1 and Notch 2 are reported to be functionally redundant
Other Names:
Neurogenic locus notch homolog protein 2, Notch-2
Structure:
Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution:
Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart. In spleen, Notch 2 is reported to be expressed primarily on B cells.
Function:
Regulate broad spectrum of various cell lineage commitment process, including myogenesis, neurogenesis, gliogenesis and lymphocyte development. Mutation of Notch2 can cause a rare genetic disorder, Alagille syndrome, with multiple defects in liver, heart and other systems.
Ligand Receptor:
Jag1,2; Delta 1,4
Antigen References:
1. Ehebauer ME et al. 2006. Biochem J 392:13 2. Shimizu K et al. 2000. Mol Cell Biology 20:18 3. Tanigaki K et al. 2007. Nature Immunol 8:451 4. Kraman M et al. 2005. FASEB J. 19:1311
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