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APC Anti-mouse Notch 2 Antibody
Material Safety Data Sheet (MSDS) APC Anti-mouse Notch 2 Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
APC Anti-mouse Notch 2 Antibody
130713 25 µg $135.00     
130714 100 µg $305.00     
Clone: HMN2-35
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Notch 2-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application
References:

Moriyama Y et al. 2008. Int J Immunology 20:763

Mouse Notch 2 transfected cells

Mouse Notch 2 transfected cells stained with HMN2-35 APC


Description:

The Notch receptors are highly conserved from invertebrates to mammals.   Notch 1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors.  The Notch1 and Notch 2 are reported to be functionally redundant

Other Names: Neurogenic locus notch homolog protein 2, Notch-2
Structure: Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution: Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart. In spleen, Notch 2 is reported to be expressed primarily on B cells.
Function: Regulate broad spectrum of various cell lineage commitment process, including myogenesis, neurogenesis, gliogenesis and lymphocyte development. Mutation of Notch2 can cause a rare genetic disorder, Alagille syndrome, with multiple defects in liver, heart and other systems.
Ligand Receptor: Jag1,2; Delta 1,4
Antigen
References:

1. Ehebauer ME et al. 2006. Biochem J 392:13
2. Shimizu K et al. 2000. Mol Cell Biology 20:18
3. Tanigaki K et al. 2007. Nature Immunol 8:451
4. Kraman M et al. 2005. FASEB J. 19:1311

APC Armenian Hamster IgG Isotype CtrlHTK888FC,ICFC
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC,ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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