The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
Moriyama Y et al. 2008. Int J Immunology 20:763
Mouse Notch 2 transfected cells stained with HMN2-35 APC
Description:
The Notch receptors are highly conserved from invertebrates to mammals.Notch 1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors.The Notch1 and Notch 2 are reported to be functionally redundant
Other Names:
Neurogenic locus notch homolog protein 2, Notch-2
Structure:
Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution:
Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart. In spleen, Notch 2 is reported to be expressed primarily on B cells.
Function:
Regulate broad spectrum of various cell lineage commitment process, including myogenesis, neurogenesis, gliogenesis and lymphocyte development. Mutation of Notch2 can cause a rare genetic disorder, Alagille syndrome, with multiple defects in liver, heart and other systems.
Ligand Receptor:
Jag1,2; Delta 1,2
Antigen References:
1. Ehebauer ME et al. 2006. Biochem J 392:13 2. Shimizu K et al. 2000. Mol Cell Biology 20:18 3. Tanigaki K et al. 2007. Nature Immunol 8:451 4. Kraman M et al. 2005. FASEB J. 19:1311
