Antibodies
Login
Search   
APC anti-mouse Ly-6G Antibody
APC anti-mouse Ly-6G Antibody
127613 25 µg $90.00     
127614 100 µg $285.00     
Product Details
Clone: 1A8
Isotype: Rat IgG2a, κ
Isotype Control:APC Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: Ly-6G transfected EL-4J cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
Notes:

While IA8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Additional reported applications (for the relevant formats) include: depletion.
Application
References:

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed.

C57BL/6 bone marrow cells stained
C57BL/6 bone marrow cells stained with 1A8 APC


Compare all formats



Description:

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in the bone marrow and granulocytes in the periphery.
Other Names: Ly6G
Structure: A 21-35 kD GPI-anchorded membrane protein
Distribution: Expressed on majority of myeloid cells in the bone marrow and granulocytes in the periphery. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
Antigen
References:

Fleming TJ, et al. 1993. J. Immunol. 151:2399.
GeneID: 546644
Latest Publications: View the latest Ly-6G articles on HighwirePress.com
UniProt: View information about Ly-6G on UniProt.org
Keywords: APC anti-mouse Ly-6G, 1A8, APC, Ly6G, Mouse, Immunology, Antibodies
Related Products
DescriptionCloneApplications
APC Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC
TruStain fcX™ (anti-mouse CD16/32)93 FC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-mouse Ly-6G
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)

  • Biotin anti-mouse Ly-6G
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 1A8 biotin, followed by Sav-PE (gated on myeloid cells)

  • FITC anti-mouse Ly-6G
    BALB/cmouse bone marrow cells stained

    BALB/cmouse bone marrow cells stained with 1A8 FITC (gated on myeloid cells)

  • PE anti-mouse Ly-6G
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 1A8 PE (gated on myeloid cells)

  • Alexa Fluor® 647 anti-mouse Ly-6G
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells stained with 1A8 Alexa Fluor® 647

  • Pacific Blue™ anti-mouse Ly-6G
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 1A8 Pacific Blue™

  • APC anti-mouse Ly-6G
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 1A8 APC

  • PerCP/Cy5.5 anti-mouse Ly-6G
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 1A8 PerCP/Cy5.5

  • PE/Cy7 anti-mouse Ly-6G
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 1A8 PE/Cy7

  • Alexa Fluor® 700 anti-mouse Ly-6G
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 1A8 Alexa Fluor® 700

  • APC/Cy7 anti-mouse Ly-6G
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with 1A8 APC/Cy7

  • Brilliant Violet 421™ anti-mouse Ly-6G
    C57BL/6 mouse bone marrow cells

    C57BL/6 mouse bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.

  • Brilliant Violet 570™ anti-mouse Ly-6G
    C57BL/6 bone marrow cells were

    C57BL/6 bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 570™ (filled histogram) or rat IgG2a, κ Brilliant Violet 570™ isotype control (open histogram). Data shown was gated on myeloid cell population.

x
Copyright © 2011 BioLegend, Inc. All rights reserved.