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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).
Application References:
1. Wall KA, et al. 1983. J. Immunol. 131:1056. (FC) 2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC) 3. Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF) 4. Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF) 5. Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC) 6. Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF) 7. Nakagawa TY, et al. 1999. Immunity 10:207. (IP) 8. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed
C57BL/6 mouse splenocytes stained with AF6-120.1 APC
The AF6-120.1 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice, and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2k and H-2u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).
Other Names:
MHC class II
Structure:
MHC class II
Distribution:
B and activated T cells, APCs of H-2b mice
Function:
Antigen presentation
Ligand Receptor:
CD3/TCR, CD4
Antigen References:
1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
APC anti-mouse I-Ab, AF6-120.1, APC, MHC class II, Mouse b haplotype, Cross-reacts with H-2k and H-2u haplotypes. Does not react with other haplotypes (e.g., d, f, q, r, s)., Immunology, Antibodies
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with biotinylated AF6-120.1, followed by Sav-PE
FITC anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 FITC
PE anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 PE
Purified anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with purified AF6-120.1, followed by anti-mouse IgG2a FITC
Alexa Fluor® 488 anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 Alexa Fluor® 647
PerCP/Cy5.5 anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 PerCP/Cy5.5
APC anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 APC
PE/Cy7 anti-mouse I-Ab
C57BL/6 splenocytes stained with AF6-120.1 PE/Cy7
Pacific Blue™ anti-mouse I-Ab
C57BL/6 splenocytes stained with AF6-120.1 Pacific Blue™
