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APC anti-mouse/human CD44 Antibody
APC anti-mouse/human CD44 Antibody
103011 25 µg $65.00 Qty:
    
103012 100 µg $185.00 Qty:
    

Product Details

 
Clone: IM7 (See other available formats)
Isotype: Rat IgG2b, κ
Isotype Control:APC Rat IgG2b, κ Isotype Ctrl
Reactivity: Mouse, Human, Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline)
Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The CD44 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation
Laser:
Red Laser (633 nm)
COA:
Enter Lot#:   
Application
Notes:

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103046) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application
References:

  Publication Library
APC anti-mouse/human CD44 Antibody IM7 Image 1
C57BL/6 mouse splenocytes stained with IM7 APC


Compare all formats

See APC spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Other Names: Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Structure: Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution: All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
Function: Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand Receptor: Hyaluronan, MIP-1β, fibronectin, collagen
Antigen
References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

GeneID: 12505
960
Latest Publications: View the latest CD44 articles on HighwirePress.com
UniProt: View information about CD44 on UniProt.org
Keywords: APC anti-mouse/human CD44, IM7, APC, Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24, Mouse, Human, Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline), Flow Cytometry, Immunology, Antibodies


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Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-mouse/human CD44IM7FC, IF, IHC
Alexa Fluor® 594 anti-mouse/human CD44IM7FC, IF
Alexa Fluor® 647 anti-mouse/human CD44IM7FC, IF, IHC
Alexa Fluor® 700 anti-mouse/human CD44IM7FC
APC/Cy7 anti-mouse/human CD44IM7FC
Biotin anti-mouse/human CD44IM7FC
Brilliant Violet 421™ anti-mouse/human CD44IM7FC
Brilliant Violet 510™ anti-mouse/human CD44IM7FC
Brilliant Violet 570™ anti-mouse/human CD44IM7FC
Brilliant Violet 605™ anti-mouse/human CD44IM7FC
Brilliant Violet 650™ anti-mouse/human CD44IM7FC
Brilliant Violet 711™ anti-mouse/human CD44IM7FC
Brilliant Violet 785™ anti-mouse/human CD44IM7FC
FITC anti-mouse/human CD44IM7FC
LEAF™ Purified anti-mouse/human CD44IM7FC, ELISA, IHC, IP, CMCD, Stim, CyTOF®
Pacific Blue™ anti-mouse/human CD44IM7FC
PE anti-mouse/human CD44IM7FC
PE/Cy5 anti-mouse/human CD44IM7FC
PE/Cy7 anti-mouse/human CD44IM7FC
PE/Dazzle™ 594 anti-mouse/human CD44IM7FC
PerCP anti-mouse/human CD44IM7FC
PerCP/Cy5.5 anti-mouse/human CD44IM7FC
Purified anti-mouse/human CD44IM7FC, ELISA, IHC, IP, CMCD, Stim, CyTOF®
Purified anti-mouse/human CD44 (MaxPar® Ready)IM7FC, CyTOF®
Ultra-LEAF™ Purified anti-mouse/human CD44IM7FC, ELISA, IHC, IP, CMCD, Stim, CyTOF®


Related Pathways

Pathway
CD4 and CD8 T-Cell Lineage
Gamma-Delta T cells
Stem Cells and Development
T Follicular Helper Cell Pathway
TH1 Pathway (Cellular Immune Response)
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Mouse Immunology > Basophils > CD44
Immunology > Non-Human Primate Immunology > CD44
Immunology > Mouse Immunology > T Regulatory Cells (TReg) > CD44
Immunology > Human Immunology > Basophils > CD44
Immunology > Mouse Immunology > Adhesion Molecules > CD44
Immunology > Human Immunology > Stem Cells > CD44 (Hermes, Pgp-1)
Immunology > Human Immunology > T Regulatory Cells (TReg) > CD44 (Hermes, Pgp-1)
Immunology > Mouse Immunology > Stem Cells > CD44 (Hermes, Pgp-1)
Cell Biology > Cell Adhesion > Hermes (CD44, Pgp-1)
Immunology > Mouse Immunology > CD and Related Molecules > CD44 > IM7
Immunology > Mouse Immunology > Th1/Th2 > CD44 > IM7
Immunology > Mouse Immunology > Th17 > CD44 > IM7
Immunology > Mouse Immunology > Costimulatory Molecules > CD44 > IM7
Immunology > Human Immunology > CD and Related Molecules > CD44 (Hermes, Pgp-1) > IM7
Immunology > Human Immunology > Adhesion Molecules > CD44 (Hermes) > IM7

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 2 Revision Date: 2014-09-11
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse/human CD44
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with IM7 APC

  • Biotin anti-mouse/human CD44
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with biotinylated CD44 (clone IM7) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE.

  • FITC anti-mouse/human CD44
    C57BL/6 splenocytes stained with IM7

    C57BL/6 splenocytes stained with IM7 FITC

    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IM7 FITC

  • LEAF™ Purified anti-mouse/human CD44
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with LEAF™ purified IM7, followed by anti-rat IgG FITC

  • PE/Cy5 anti-mouse/human CD44
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with IM7 PE/Cy5

  • Purified anti-mouse/human CD44
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified IM7, followed by anti-rat IgG FITC

  • Brilliant Violet 605™ anti-mouse/human CD44
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Brilliant Violet 605™ (filled histogram) or rat IgG2b, κ Brilliant Violet 605™ isotype control (open histogram).

  • PE anti-mouse/human CD44
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with IM7 PE

    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IM7 PE

  • Alexa Fluor® 488 anti-mouse/human CD44
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with IM7 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-mouse/human CD44
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with IM7 Alexa Fluor® 647

    MDA-MB231 breast cancer cell line

    MDA-MB231 breast cancer cell line was stained with 5 µg/mL anti-human CD44 Alexa Fluor® 647 and nuclear counterstained with DAPI. Images were acquired with a TE300 fluorescence microscope with a 20x objective. Data provided by: Er Liu and John Nolan, La Jolla Bioengineering Institute

  • Pacific Blue™ anti-mouse/human CD44
    C57BL/6 Mouse Splenocytes stained with

    C57BL/6 Mouse Splenocytes stained with IM7 Pacific Blue™

  • Alexa Fluor® 700 anti-mouse/human CD44
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with IM7 Alexa Fluor® 700

  • PE/Cy7 anti-mouse/human CD44
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with IM7 PE/Cy7

  • APC/Cy7 anti-mouse/human CD44
    C57BL/6 splenocytes stained with IM7

    C57BL/6 splenocytes stained with IM7 APC/Cy7

  • PerCP/Cy5.5 anti-mouse/human CD44
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with IM7 PerCP/Cy5.5

  • PerCP anti-mouse/human CD44
    C57BL/6 splenocytes stained with IM7

    C57BL/6 splenocytes stained with IM7 PerCP

  • Brilliant Violet 421™ anti-mouse/human CD44
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).

  • Brilliant Violet 570™ anti-mouse/human CD44
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Brilliant Violet 570™ (filled histogram) or rat IgG2b, κ Brilliant Violet 570™ isotype control (open histogram).

  • Brilliant Violet 785™ anti-mouse/human CD44
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Brilliant Violet 785™.

  • Brilliant Violet 510™ anti-mouse/human CD44
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Brilliant Violet 510™.

  • Ultra-LEAF™ Purified anti-mouse/human CD44
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified CD44 (clone IM7), followed by anti-rat IgG FITC.

  • Brilliant Violet 650™ anti-mouse/human CD44
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Brilliant Violet 650™ (filled histogram) or rat IgG2b, κ Brilliant Violet 650™ isotype control (open histogram).

  • Purified anti-mouse/human CD44 (MaxPar® Ready)
    C57BL/6 mouse splenocytes (blue) and

    C57BL/6 mouse splenocytes (blue) and human Jurkat T cells (red) stained with 171Yb-anti-CD44 (IM7). Data provided by DVS Sciences.

  • Alexa Fluor® 594 anti-mouse/human CD44
    NIH/3T3 cells were fixed with

    NIH/3T3 cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/ml of anti-human/mouse CD44 (clone IM7) Alexa Fluor® 594 (red) in blocking buffer for 3 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40X objective.

    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Alexa Fluor® 594 (filled histogram). The data was acquired by BD LSRFortessa™ cell analyzer equipped with Yellow-Green Laser (561 nm).

    HUVEC cells were fixed with

    HUVEC cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/ml of anti-human/mouse CD44 (clone IM7) Alexa Fluor® 594 (red) in blocking buffer for 3 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40X objective.

  • PE/Dazzle™ 594 anti-mouse/human CD44
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) PE/Dazzle 594™ (filled histogram) or rat IgG2b, κ PE/Dazzle 594™ isotype control (open histogram).

  • Brilliant Violet 711™ anti-mouse/human CD44
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Brilliant Violet 711™ (filled histogram) or rat IgG2b, κ Brilliant Violet 711™ isotype control (open histogram).

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