The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality Tested
IP1* (for relevant formats)- *This application has been published in the literature
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Kraus M., et al. 2001.J. Exp. Med. 194:455
C57BL/6 splenocytes stained with B220 (RA3-6B2) PE and F11-172 APC
Description:
Mouse CD79a (Ig α chain) is a 47 kDa type I integral membrane protein, known as mb-1 or Ig α. It forms a heterodimer with CD79b (Ig β chain). The CD79a and CD79b heterodimers are associated with surface IgM to form the B-cell receptor (BCR) that is necessary for signal transduction via the BCR in mature B cells. CD79a may play a role in mediating the transport of IgM to the cell surface. Clone F11-172 antibody recognizes the 47 kDa membrane glycoprotein present on B lymphocytes.
Other Names:
Mb-1, Igα
Structure:
A 47 kDa membrane glycoprotein which associates with CD79b to form part of B-cell receptor complex.
Distribution:
CD79a is expressed on B cells at various differentiation stages, from pre-B cell to plasma cells. It is expressed probably before expression of cytoplasmic µu chain.
Function:
Mediating the transport of IgM to the cell surface.
Ligand Receptor:
CD79a/CD79b heterodimers are associated with surface IgM to form B cell receptor complex (BCR) to recognize specific antigen.
Antigen References:
1. Schamel W., et al. 2000. Immunity. 13:5 2. Taddie, J.A., et al.1994. J. Biol. Chem. 269:13529
