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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting6, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections2,4,5,6.
Application References:
1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. (FC) 2. Knowles HJ, et al. 2004. Circ. Res. 95:162. (IHC) 3. Trempus CS, et al. 2003. J. Invest. Dermatol. 120:501. 4. Winding B, et al. 2002. Clin. Cancer Res. 8:1932. (IHC) 5. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC) 6. Kairaitis LK, et al. 2005. Am. J. Physiol. Renal. Physiol. 288:F198. (IHC, WB) 7. Ao A, et al. 2008. P. Natl. Acad. Sci. USA 105:7821. PubMed
CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have been reported to be generated by alternative splicing. This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.
Other Names:
Mucosialin
Structure:
Type I membrane protein, 75-120 kD, highly glycosylated; two isoforms reported
Distribution:
Hematopoietic progenitors, brain, testis, endothelial cells; low expression in thymus, spleen, and bone marrow
Function:
Possible adhesion molecule thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Presents carbohydrate ligands to selectins.
Ligand Receptor:
L-selectin, other selectins
Antigen References:
1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. 2. Brown J, et al. 1991. Int. Immunol. 3:175. 3. Suda J, et al. 1992. Blood 79:2288. 4. Baumhueter S, et al. 1994. Blood 84:2554.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD34
Mouse NIH/3T3 cell line stained with purified MEC14.7, followed with biotinylated anti-rat IgG and Sav-PE
Biotin anti-mouse CD34
Mouse NIH/3T3 cell line stained with biotinylated MEC14.7, followed by Sav-PE
PE anti-mouse CD34
Mouse NIH/3T3 cell line stained with MEC14.7 PE
Alexa Fluor® 647 anti-mouse CD34
Mouse NIH/3T3 cell line stained with MEC14.7 Alexa Fluor® 647
PE/Cy5 anti-mouse CD34
Mouse NIH/3T3 cell line stained with MEC14.7 PE/Cy5
APC anti-mouse CD34
NIH3T3 cells stained with MEC14.7APC
Brilliant Violet 421™ anti-mouse CD34
Mouse fibroblast cell line NIH/3T3 was stained with CD34 (clone MEC14.7) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
