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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy and functional assay2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays.
Application References:
1. Blasius AL, et al. 2006. J. Immunol. 177:3260. 2. Schliemann C, et al. 2010. Blood 115:736. (FA, IF)
C57BL/6 splenocytes stained with 927 APC and CD45R/B220 (RA3-6B2) PE
C57BL/6 splenocytes stained with rat IgG2b APC isotype control and CD45R/B220 (RA3-6B2) PE
CD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 is a recently identified, IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.
Other Names:
BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
Structure:
Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
Distribution:
Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.
Function:
Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
Antigen References:
1. Douglas JL. et al. 2009. J Virol. 83(16):7931 2. Cao W et al. 2009. J. Exp. Med. 206(7):1603 3. Neil SJ. et al. 2008. Nature 451:425
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD317 (BST2, PDCA-1)
C57BL/6 splenocytes stained with RA3-6B2 (B220) APC and 927 PE
PE anti-mouse CD317 (BST2, PDCA-1)
C57BL/6 splenocytes stained with RA3-6B2 (B220) APC and 927 PE
Alexa Fluor® 488 anti-mouse CD317 (BST2, PDCA-1)
C57BL/6 splenocytes stained with RA3-6B2 (B220) APC and 927 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD317 (BST2, PDCA-1)
SJL mouse splenocytes stained with 927 Alexa Fluor® 647 and CD45R/B220 (RA3-6B2) PE
SJL mouse splenocytes stained with rat IgG2b isotype control Alexa Fluor® 647 and CD45R/B220 (RA3-6B2) PE
APC anti-mouse CD317 (BST2, PDCA-1)
C57BL/6 splenocytes stained with 927 APC and CD45R/B220 (RA3-6B2) PE
C57BL/6 splenocytes stained with rat IgG2b APC isotype control and CD45R/B220 (RA3-6B2) PE
Pacific Blue™ anti-mouse CD317 (BST2, PDCA-1)
C57BL/6 splenocytes stained with 927 Pacific Blue™ and CD45R/B220 (RA3-6B2) PE
C57BL/6 splenocytes stained with rat IgG2b Pacific Blue™ isotype control and CD45R/B220 (RA3-6B2) PE
Biotin anti-mouse CD317 (BST2, PDCA-1)
C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) APC and biotinylated 927, followed by Sav-PE
C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) APC and biotinylated rat IgG2b isotype control, followed by Sav-PE
FITC anti-mouse CD317 (BST2, PDCA-1)
C57BL/6 splenocytes stained with 927 FITC and CD45R/B220 (RA3-6B2) APC
C57BL/6 splenocytes stained with rat IgG2b FITC isotype control and CD45R/B220 (RA3-6B2) APC
