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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The LEAF™Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 101906).
Application References:
1. Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block) 2. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block) 3. Mushaben EM, et al. 2011. J. Immunol. 187:5756. PubMed.
ConA-stimulated BALB/c splenocytes (Day 3) stained with 3C7 APC
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Other Names:
IL-2Rα, Ly-43, p55, Tac
Structure:
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution:
Activated T and B cells, thymocyte subset, pre-B cells, T regulatory cells
Function:
IL-2 receptor, signal transduction
Ligand Receptor:
IL-2
Antigen References:
1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann TA. 1991. J. Biol. Chem. 266:2681. 3. Read S, et al. 2000. J. Exp. Med. 192:295. 4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse CD25
Con A-activated (3days) C57BL/6 mouse splenocytes stained with 3C7 PE
Purified anti-mouse CD25
Con A-activated (3days) C57BL/6 mouse splenocytes stained with purified 3C7,followed by anti-rat IgG FITC
FITC anti-mouse CD25
ConA-stimulated BALB/c splenocytes (Day 3) stained with 3C7 FITC
APC anti-mouse CD25
ConA-stimulated BALB/c splenocytes (Day 3) stained with 3C7 APC
PerCP/Cy5.5 anti-mouse CD25
Con A-stimulated C57BL/7 splenocytes (Day 3) stained with 3C7 PerCP/Cy5.5
