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APC anti-mouse CD21/CD35 (CR2/CR1) Antibody
APC anti-mouse CD21/CD35 (CR2/CR1) Antibody
123411 25 µg $100.00     
123412 100 µg $275.00     
Product Details
Clone: 7E9
Isotype: Rat IgG2a, κ
Isotype Control:APC Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
References:

1. Boackle S, et al. 2001 Immunity 15:775.
2. Zane NA, et al. 2012. J. Immunol. 188:3009. PubMed.

C57BL/6 mouse splenocytes stained with
C57BL/6 mouse splenocytes stained with 7E9 APC


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Description:

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.
Other Names: CR2/CR1
Structure: 145 kD/190 kD type I transmembrane glycoprotein
Distribution: B cells, follicular dendritic cells
Function: Signal transduction
Ligand Receptor: Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Antigen
References:

1. Kozono Y, et al. 1998. J. Immunol. 160:1562.
2. Shimizu I, et al. 2007. Blood 109:1773.
3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.
GeneID: 12902
12946
Latest Publications: View the latest CD21/35 articles on HighwirePress.com
UniProt: View information about CD21/35 on UniProt.org
Keywords: APC anti-mouse CD21/CD35 (CR2/CR1), 7E9, APC, CR2/CR1, Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • PE/Cy7 anti-mouse CD21/CD35 (CR2/CR1)
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 7E9 PE/Cy7

  • APC/Cy7 anti-mouse CD21/CD35 (CR2/CR1)
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 7E9 APC/Cy7 and CD3 PE

  • Purified anti-mouse CD21/CD35 (CR2/CR1)
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified 7E9, followed by anti-rat IgG FITC

  • LEAF™ Purified anti-mouse CD21/CD35 (CR2/CR1)
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with LEAF™ purified 7E9, followed by anti-rat IgG FITC

  • Biotin anti-mouse CD21/CD35 (CR2/CR1)
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with biotinylated 7E9, followed by Sav-PE

  • FITC anti-mouse CD21/CD35 (CR2/CR1)
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 7E9 FITC

  • PE anti-mouse CD21/CD35 (CR2/CR1)
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 7E9 PE

  • APC anti-mouse CD21/CD35 (CR2/CR1)
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 7E9 APC

  • Pacific Blue™ anti-mouse CD21/CD35 (CR2/CR1)
    C57BL/6 splenocytes stained with 7E9

    C57BL/6 splenocytes stained with 7E9 Pacific Blue™

  • PerCP/Cy5.5 anti-mouse CD21/CD35 (CR2/CR1)
    C57BL/6 splenocytes stained with 7E9

    C57BL/6 splenocytes stained with 7E9 PerCP/Cy5.5

  • Brilliant Violet 421™ anti-mouse CD21/CD35 (CR2/CR1)
    C57BL/6 splenocytes stained with CD21/CD35

    C57BL/6 splenocytes stained with CD21/CD35 (clone 7E9) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).

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