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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use licenses cannot be guaranteed from the University of California.
Application References:
1. McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC) 2. Vance RE, et al. 2002. P. Natl. Acad. Sci. USA 99:868. (FC)
C57BL/6 mouse splenocytes were stained with NK1.1 (clone PK136) PE and CD159a (NKG2AB6, clone 16A11, top) APC or mouse IgG2b, κ APC isotype control (bottom).
CD159a, also known as NKG2A or KLRC1 (killer cell lectin-like receptor subfamily C, member 1), is a 43 kD type II transmembrane protein with extracellular C-type lectin domains. It belongs to the killer cell lectin-like receptor family also known as the NKG2 family. It is expressed on NK and NKT cells and activated CD8+ T cells. NKG2A binds to non-classical MHC class I molecule Qa-1 and causes inhibition of NK cell-mediated target-cell lysis.
Other Names:
KLRC1, NKG2B
Structure:
Type II transmembrane protein with extracelluar C-type lectin domains, complexed with CD94; 43 kD
Distribution:
NK cells and NK T cells from C57BL/6 mouse
Function:
Inhibitory activity via ITIMs
Ligand Receptor:
Non-classical MHC-I molecule Qa-1
Antigen References:
1. Vance RE, et al. 1998. J. Exp. Med. 188:1841. 2. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755. 3. Sivakumar PV, et al. 1999. J. Immunol. 162:6976. 4. Silver ET, et al. 1999. Immunogenetics 49:727. 5. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse CD159a (NKG2AB6)
C57BL/6 mouse splenocytes were stained with NK1.1 Alexa Fluor® 647 and CD159a (NKG2AB6) (clone 16A11) PE (top) or mouse IgG2b, κ PE isotype control (bottom).
Purified anti-mouse CD159a (NKG2AB6)
C57BL/6 mouse splenocytes were stained with NK1.1 Alexa Fluor® 647 and purified CD159a (NKG2AB6) (clone 16A11) (top) or mouse IgG2b, κ isotype control (bottom), followed by anti-mouse IgG2b FITC.
Biotin anti-mouse CD159a (NKG2AB6)
C57BL/6 mouse splenocytes were stained with anti-mouse NK1.1 (clone PK136) APC and biotinylated anti-mouse CD159a (NKG2AB6, clone 16A11, top) or mouse IgG2b, κ isotype control (bottom), followed by Sav-PE.
APC anti-mouse CD159a (NKG2AB6)
C57BL/6 mouse splenocytes were stained with NK1.1 (clone PK136) PE and CD159a (NKG2AB6, clone 16A11, top) APC or mouse IgG2b, κ APC isotype control (bottom).
