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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and immunofluorescent staining2,3.
Application References:
1. Jalkanen M, et al. 1985. J. Cell Biol. 101:976. (FC) 2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF) 3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) APC (top) or rat IgG2a, κ APC isotype control (bottom). Data shown is from gated on lymphoid cell population.
CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.
Other Names:
Syndecan-1
Structure:
Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown is from gated on myeloid population.
PE anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown is from gated on myeloid population.
APC anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) APC (top) or rat IgG2a, κ APC isotype control (bottom). Data shown is from gated on lymphoid cell population.
C57BL/6 mouse bone marrow cells were stained with CD19 APC and CD138 (clone 281-2) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom). Data shown is from gated on lymphoid cell population.
