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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections3 and paraffin-embedded sections5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 100208).
Application References:
1. Rodriguez-Gago M, et al. 2001. Transplantation. 72:503. 2. Lehmann FS, et al. 2002. Am. J. Physiol. Gastrointest. Liver. Physiol. 283:G481. (FC) 3. Bordignon M, et al. 2008. Mol. Med. Rep. 1:485. (IHC) 4. Conrad M, et al. 2007. Cytom. Part A 71A:925. (FC) 5. Pollinger B, et al. 2011. J. Immunol. 186:2602. (IHC)
Human peripheral blood lymphocytes stained with CD3 PE and B1 APC
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ is involved in the recognition of certain bacterial, self-CD1 molecule, and tumor antigens bound to MHC class I. The γ/δ TCR associates with CD3 and is expressed on a subset of T cells found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, innate immune response for some tumor cells, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.
Other Names:
T cell receptor γ/δ, γ/δ TCR, TCR-γ/δ
Structure:
Ig superfamily, associates with CD3 complex
Distribution:
T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum
Function:
Antigen recognition
Ligand Receptor:
Some bacterial or tumor antigens bound MHC class I, CD1 molecule
Antigen References:
1. Lanier LL, et al. 1987. J. Clin. Immunol. 7:429. 2. Spencer J, et al. 1989. Eur. J. Immunol. 19:1335. 3. Uyemura K, et al. 1991. J. Exp. Med. 174:683. 4. Spada FM, et al. 2000. J. Exp. Med. 191:907.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human TCR γ/δ
Human peripheral blood lymphocytes stained with purified B1, followed by anti-mouse IgG FITC
LEAF™ Purified anti-human TCR γ/δ
Human peripheral blood lymphocytes stained with LEAF™ purified B1, followed by anti-mouse IgG FITC
Biotin anti-human TCR γ/δ
Human peripheral blood lymphocytes stained with CD3 APC and biotinylated B1, followed by Sav-PE
PE anti-human TCR γ/δ
Human peripheral blood lymphocytes stained with CD3 (UCHT1) APC and B1 PE
APC anti-human TCR γ/δ
Human peripheral blood lymphocytes stained with CD3 PE and B1 APC
Alexa Fluor® 647 anti-human TCR γ/δ
Human peripheral blood lymphocytes stained with CD3 PE and B1 Alexa Fluor® 647
