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APC anti-human TCR α/β Antibody
Cat. # Size Price  

306717 25 tests $95.00
    
306718 100 tests $210.00
    
Product Details

Clone: IP26 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:APC Mouse IgG1, κ Isotype Ctrl (FC)
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
Excitation
Laser:
Red Laser (633 nm)
Application
Notes:
Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone.  Other anti-CD3 clones may compete out the binding of IP26.  
Application
References:

  Publication Library
 Image 1
Human peripheral blood lymphocytes stained with IP26 APC


Compare all formats

See APC spectral data





 


Antigen Details

Description:

The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.

Other Names: α/β TCR, TCR α/β
Structure: Ig superfamily, with CD3 forms CD3/TCR complex
Distribution: T cells, thymocytes
Function: Antigen recognition, T cell activation
Ligand Receptor: Peptide bound to MHC
Antigen
References:
1. Marchalonis J, et al. 2002. J. Mol. Recognit. 15:260.
GeneID: 6955
6957
UniProt: View information about TCR alpha/beta on UniProt.org
Keywords: APC anti-human TCR α/β, IP26, APC, α/β TCR, TCR α/β, Human, Flow Cytometry, Immunology, Antibodies
Technical Data Sheet (pdf)
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated IP26, followed by Sav-PE

  • FITC anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 FITC

  • PE anti-human α/β T Cell Receptor
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 PE

  • PE/Cy5 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 PE/Cy5

  • Purified anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified IP26, then detected with anti-mouse IgG FITC

  • PE/Cy7 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD19 (HIB19) FITC and IP26 PE/Cy7 (top) or mouse IgG1, κ PE/Cy7 isotype control (bottom)





  • Alexa Fluor® 488 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 Alexa Fluor® 647

  • Pacific Blue™ anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 Pacific Blue™

  • APC anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 APC

  • Brilliant Violet 421™ anti-human TCR α/β
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-human TCRα/β (clone IP26) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

  • PerCP/Cy5.5 anti-human TCR α/β
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-human TCR α/β (clone IP26) PerCP/Cy5.5 (filled histogram) or mouse IgG1, κ PerCP/Cy5.5 isotype control (open histogram).

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