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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Human prostatic adenocarcinoma cell line LNCaP cells were stained with PSMA (clone LNI-17) APC (filled histogram) or mouse IgG1 APC isotype control (open histogram).
PSMA (prostate-specific membrane antigen) is a type II transmembrane zinc metallopeptidase, belonging to the M28 peptidase family. It possesses hydrolyzing enzyme activities and is also known as FOLH1 (folate hydrolase 1), GCP2 (glutamate carboxypeptidase II), NAALADase I (N-acetylated-alpha-linked acidic dipeptidase I). PSMA is expressed by normal and malignant prostate epithelial cells, by urothelial adenocarcinoma, and vasculatures of other malignancies. It is also found in the nervous system.
Type II transmembrane zinc metallopeptidase, M28 peptidase family
Distribution:
Epithelial cells in prostate, intestine and kidney tissue, nervous system
Function:
Hydrolyzing enzyme activity
Antigen References:
1. Silver DA, et al. 1997. Clin. Cancer Res. 3:81. 2. Troyer JK, et al. 1995. Int. J. Cancer. 62:552. 3. Mannweiler S, et al. 2009. Pathol. Oncol. Res. 15:167.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human PSMA (FOLH1)
Human prostatic adenocarcinoma cell line LNCaP stained with purified LNI-17 conjugated to PE
PE anti-human PSMA (FOLH1)
Human prostatic adenocarcinoma cell line LNCaP stained with LNI-17 PE
Alexa Fluor® 488 anti-human PSMA (FOLH1)
Human prostatic adenocarcinoma cell line LNCaP stained with LNI-17 Alexa Fluor® 488
APC anti-human PSMA (FOLH1)
Human prostatic adenocarcinoma cell line LNCaP cells were stained with PSMA (clone LNI-17) APC (filled histogram) or mouse IgG1 APC isotype control (open histogram).
