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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
Excitation Laser:
Red Laser (633 nm)
COA:
Human acute lymphoblastic leukemia cells HPB-ALL stained with MHN4-2 APC
Neurogenic locus notch homolog protein 4 (Notch 4) is a transmembrane heterodimer in which the extracellular domain contains 29 EGF repeats and 3 LNR repeats. The intracellular domain contains 5 ANK repeats, 1 PEST motif, and 1 RAM23 domain. Notch 4 controls many developmental processes in the cell such as differentiation, proliferation, and apoptosis. In addition, it also plays a role in vascular, renal, and hepatic development. Notch 4 is expressed by a variety of tissues such as heart, lung, placenta, liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow, and fetal liver. The Notch 4 ligands are Jagged1, Jagged2, Delta1, Delta3 and Delta4. After ligand binding, Notch 4 is cleaved by the TNF-α converting enzyme (TACE). The extracellular domain remains on the cell surface while the intracellular domain is released and translocated to the nucleus where it binds to CBF-1, leading to the expression of genes of the Hairy/Enhancer of Split (HES) and HES-related repressor protein (HERP) families.
Other Names:
Neurogenic locus notch homolog protein 4
Structure:
Transmembrane protein. Extracellular domain forms a heterodimer and contains 29 epidermal growth factor-like repeats and 3 Lin/Notch Glp repeats. The intracellular domain contains 5 CDC10/Ankryn repeats, 1 proline, glutamate, serine, threonine-rich motif,
Distribution:
Heart, lung, placenta, liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow, and fetal liver.
Function:
Controls many cell developmental processes such as differentiation, proliferation and apoptosis. Plays a role in vascular, renal, and hepatic development.
Ligand Receptor:
Jagged1, Jagged2, Delta1, Delta3, and Delta4
Interaction:
MAML1, MAML2 and MAML3, CBF-1, Smad3, and Smad4
Antigen References:
1. Katoh M and Katoh M. 2007. Int. J. Oncol. 30:247. 2. Kerbel RS. 2008. N. Engl. J. Med. 358:2039. 3. Chen VC, et al. 2008. Nat. Biotechnol. 26:1169. 4. Emuss V. 2009. PLoS Pathog. 5:e1000616. 5. Harrison H, et al. 2010. Cancer Res. 70:709.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human Notch 4
Human acute lymphoblastic leukemia cells HPB-ALL stained with purified MHN4-2 conjugated with PE
PE anti-human Notch 4
Human acute lymphoblastic leukemia cells HPB-ALL stained with MHN4-2 PE
APC anti-human Notch 4
Human acute lymphoblastic leukemia cells HPB-ALL stained with MHN4-2 APC
